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Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

Alves, Rita C ; Pimentel, Filipa B ; Nouws, Henri P. A ; Correr, Wagner ; González-García, María Begoña ; Oliveira, M. Beatriz P. P ; Delerue-Matos, Cristina

Analytical and bioanalytical chemistry, 2015-09, Vol.407 (23), p.7157-7163 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
Autor: Alves, Rita C ; Pimentel, Filipa B ; Nouws, Henri P. A ; Correr, Wagner ; González-García, María Begoña ; Oliveira, M. Beatriz P. P ; Delerue-Matos, Cristina
Assuntos: 2S Albumins, Plant - analysis ; 2S Albumins, Plant - immunology ; Allergens ; Analytical Chemistry ; Antibodies ; Antibodies, Monoclonal - immunology ; antigen-antibody reactions ; Antigens, Plant - analysis ; Antigens, Plant - immunology ; Arachis hypogaea ; Biochemistry ; Biosensing Techniques - instrumentation ; Biosensors ; Carbon ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Conductometry - instrumentation ; Deposition ; electrochemistry ; Electrodes ; Electrons ; Equipment Design ; Equipment Failure Analysis ; Food allergies ; Food allergy ; Food Analysis - instrumentation ; Food Contamination - analysis ; food matrix ; Food Safety - methods ; Food Science ; Foods ; Health aspects ; Immunoassay ; Immunoassay - instrumentation ; immunoassays ; Immunology ; Immunosensors ; Laboratory Medicine ; Mathematical analysis ; Monitoring/Environmental Analysis ; Nanoparticles ; Peanuts ; Reproducibility of Results ; Research Paper ; Sensitivity and Specificity ; Signal transduction ; silver ; Voltammetry
É parte de: Analytical and bioanalytical chemistry, 2015-09, Vol.407 (23), p.7157-7163
Notas: http://dx.doi.org/10.1007/s00216-015-8879-8
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Descrição: A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

Alves, Rita C ; Pimentel, Filipa B ; Nouws, Henri P. A ; Correr, Wagner ; González-García, María Begoña ; Oliveira, M. Beatriz P. P ; Delerue-Matos, Cristina

Berlin/Heidelberg: Springer Berlin Heidelberg

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