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The Contribution of 700,000 ORF Sequence Tags to the Definition of the Human Transcriptome

Camargo, Anamaria A. ; Helena P. B. Samaia ; Dias-Neto, Emmanuel ; Simão, Daniel F. ; Migotto, Italo A. ; Marcelo R. S. Briones ; Costa, Fernando F. ; Nagai, Maria Aparecida ; Verjovski-Almeida, Sergio ; Zago, Marco A. ; Luis Eduardo C. Andrade ; Carrer, Helaine ; Hamza F. A. El-Dorry ; Espreafico, Enilza M. ; Habr-Gama, Angelita ; Giannella-Neto, Daniel ; Goldman, Gustavo H. ; Gruber, Arthur ; Hackel, Christine ; Kimura, Edna T. ; Rui M. B. Maciel ; Suely K. N. Marie ; Elizabeth A. L. Martins ; Nóbrega, Marina P. ; Paçó-Larson, Maria Luisa ; Maria Inês M. C. Pardini ; Pereira, Gonçalo G. ; Pesquero, João Bosco ; Rodrigues, Vanderlei ; Rogatto, Silvia R. ; Ismael D. C. G. da Silva ; Sogayar, Mari C. ; Maria de Fátima Sonati ; Tajara, Eloiza H. ; Valentini, Sandro R. ; Alberto, Fernando L. ; Maria Elisabete J. Amaral ; Aneas, Ivy ; Liliane A. T. Arnaldi ; de Assis, Angela M. ; Bengtso, Mário Henrique ; Bergamo, Nadia Aparecida ; Bombonato, Vanessa ; Maria E. R. de Camargo ; Canevari, Renata A. ; Carraro, Dirce M. ; Cerutti, Janete M. ; Maria Lucia C. Corrêa ; Rosana F. R. Corrêa ; Maria Cristina R. Costa ; Curcio, Cyntia ; Paula O. M. Hokama ; Ari J. S. Ferreira ; Furuzawa, Gilberto K. ; Gushiken, Tsieko ; Ho, Paulo L. ; Kimura, Elza ; Krieger, José E. ; Luciana C. C. Leite ; Majumder, Paromita ; Marins, Mozart ; Marques, Everaldo R. ; Analy S. A. Melo ; Melo, Monica ; Mestriner, Carlos Alberto ; Miracca, Elisabete C. ; Miranda, Daniela C. ; Ana Lucia T. O. Nascimento ; Nóbrega, Francisco G. ; Élida P. B. Ojopi ; José Rodrigo C. Pandolfi ; Pessoa, Luciana G. ; Prevedel, Aline C. ; Rahal, Paula ; Rainho, Claudia A. ; Eduardo M. R. Reis ; Ribeiro, Marcelo L. ; da Rós, Nancy ; de Sá, Renata G. ; Sales, Magaly M. ; Simone Cristina Sant'anna ; Mariana L. dos Santos ; da Silva, Aline M. ; da Silva, Neusa P. ; Silva, Wilson A. ; da Silveira, Rosana A. ; Sousa, Josane F. ; Stecconi, Daniella ; Tsukumo, Fernando ; Valente, Valéria ; Soares, Fernando ; Moreira, Eloisa S. ; Nunes, Diana N. ; Correa, Ricardo G. ; Zalcberg, Heloisa ; Carvalho, Alex F. ; Luis F. L. Reis ; Brentani, Ricardo R. ; Andrew J. G. Simpson ; de Souza, Sandro J.

Proceedings of the National Academy of Sciences - PNAS, 2001-10, Vol.98 (21), p.12103-12108 [Periódico revisado por pares]

United States: National Academy of Sciences

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Título:
The Contribution of 700,000 ORF Sequence Tags to the Definition of the Human Transcriptome
Autor: Camargo, Anamaria A. ; Helena P. B. Samaia ; Dias-Neto, Emmanuel ; Simão, Daniel F. ; Migotto, Italo A. ; Marcelo R. S. Briones ; Costa, Fernando F. ; Nagai, Maria Aparecida ; Verjovski-Almeida, Sergio ; Zago, Marco A. ; Luis Eduardo C. Andrade ; Carrer, Helaine ; Hamza F. A. El-Dorry ; Espreafico, Enilza M. ; Habr-Gama, Angelita ; Giannella-Neto, Daniel ; Goldman, Gustavo H. ; Gruber, Arthur ; Hackel, Christine ; Kimura, Edna T. ; Rui M. B. Maciel ; Suely K. N. Marie ; Elizabeth A. L. Martins ; Nóbrega, Marina P. ; Paçó-Larson, Maria Luisa ; Maria Inês M. C. Pardini ; Pereira, Gonçalo G. ; Pesquero, João Bosco ; Rodrigues, Vanderlei ; Rogatto, Silvia R. ; Ismael D. C. G. da Silva ; Sogayar, Mari C. ; Maria de Fátima Sonati ; Tajara, Eloiza H. ; Valentini, Sandro R. ; Alberto, Fernando L. ; Maria Elisabete J. Amaral ; Aneas, Ivy ; Liliane A. T. Arnaldi ; de Assis, Angela M. ; Bengtso, Mário Henrique ; Bergamo, Nadia Aparecida ; Bombonato, Vanessa ; Maria E. R. de Camargo ; Canevari, Renata A. ; Carraro, Dirce M. ; Cerutti, Janete M. ; Maria Lucia C. Corrêa ; Rosana F. R. Corrêa ; Maria Cristina R. Costa ; Curcio, Cyntia ; Paula O. M. Hokama ; Ari J. S. Ferreira ; Furuzawa, Gilberto K. ; Gushiken, Tsieko ; Ho, Paulo L. ; Kimura, Elza ; Krieger, José E. ; Luciana C. C. Leite ; Majumder, Paromita ; Marins, Mozart ; Marques, Everaldo R. ; Analy S. A. Melo ; Melo, Monica ; Mestriner, Carlos Alberto ; Miracca, Elisabete C. ; Miranda, Daniela C. ; Ana Lucia T. O. Nascimento ; Nóbrega, Francisco G. ; Élida P. B. Ojopi ; José Rodrigo C. Pandolfi ; Pessoa, Luciana G. ; Prevedel, Aline C. ; Rahal, Paula ; Rainho, Claudia A. ; Eduardo M. R. Reis ; Ribeiro, Marcelo L. ; da Rós, Nancy ; de Sá, Renata G. ; Sales, Magaly M. ; Simone Cristina Sant'anna ; Mariana L. dos Santos ; da Silva, Aline M. ; da Silva, Neusa P. ; Silva, Wilson A. ; da Silveira, Rosana A. ; Sousa, Josane F. ; Stecconi, Daniella ; Tsukumo, Fernando ; Valente, Valéria ; Soares, Fernando ; Moreira, Eloisa S. ; Nunes, Diana N. ; Correa, Ricardo G. ; Zalcberg, Heloisa ; Carvalho, Alex F. ; Luis F. L. Reis ; Brentani, Ricardo R. ; Andrew J. G. Simpson ; de Souza, Sandro J.
Assuntos: Biological Sciences ; Complementary DNA ; Datasets ; Deoxyribonucleic acid ; DNA ; Expressed Sequence Tags ; Genes ; Genetics ; Genome, Human ; Genomes ; Genomics ; Human genome ; Humans ; Messenger RNA ; Nucleotides ; Open Reading Frames ; Reverse transcriptase polymerase chain reaction ; Sequencing ; Transcription, Genetic ; transcriptome
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2001-10, Vol.98 (21), p.12103-12108
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
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Edited by Robert H. Waterston, Washington University School of Medicine, St. Louis, MO, and approved August 2, 2001
Descrição: Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data from the central protein coding portion of transcripts. We generated a total of 696,745 ORESTES sequences from 24 human tissues and used a subset of the data that correspond to a set of 15,095 full-length mRNAs as a means of assessing the efficiency of the strategy and its potential contribution to the definition of the human transcriptome. We estimate that ORESTES sampled over 80% of all highly and moderately expressed, and between 40% and 50% of rarely expressed, human genes. In our most thoroughly sequenced tissue, the breast, the 130,000 ORESTES generated are derived from transcripts from an estimated 70% of all genes expressed in that tissue, with an equally efficient representation of both highly and poorly expressed genes. In this respect, we find that the capacity of the ORESTES strategy both for gene discovery and shotgun transcript sequence generation significantly exceeds that of conventional ESTs. The distribution of ORESTES is such that many human transcripts are now represented by a scaffold of partial sequences distributed along the length of each gene product. The experimental joining of the scaffold components, by reverse transcription-PCR, represents a direct route to transcript finishing that may represent a useful alternative to full-length cDNA cloning.
Editor: United States: National Academy of Sciences
Idioma: Inglês

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The Contribution of 700,000 ORF Sequence Tags to the Definition of the Human Transcriptome

United States: National Academy of Sciences

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