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M Phase Phosphoprotein 1 Is a Human Plus-end-directed Kinesin-related Protein Required for Cytokinesis

Abaza, Aouatef ; Soleilhac, Jean-Marc ; Westendorf, Joanne ; Piel, Matthieu ; Crevel, Isabelle ; Roux, Aurélien ; Pirollet, Fabienne

The Journal of biological chemistry, 2003-07, Vol.278 (30), p.27844-27852 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
M Phase Phosphoprotein 1 Is a Human Plus-end-directed Kinesin-related Protein Required for Cytokinesis
Autor: Abaza, Aouatef ; Soleilhac, Jean-Marc ; Westendorf, Joanne ; Piel, Matthieu ; Crevel, Isabelle ; Roux, Aurélien ; Pirollet, Fabienne
Assuntos: Adenosine Triphosphatases ; Adenosine Triphosphatases - metabolism ; Amino Acid Sequence ; Animals ; Cell Cycle Proteins ; Cell Division ; Cell Division - physiology ; Cell Line ; Cell Movement ; Cells, Cultured ; Cellular Biology ; Cloning, Molecular ; Cytoplasm ; Cytoplasm - metabolism ; DNA, Complementary ; DNA, Complementary - metabolism ; Electrophoresis, Polyacrylamide Gel ; Epitopes ; Flow Cytometry ; G2 Phase ; Gene Library ; Genome ; Green Fluorescent Proteins ; HeLa Cells ; Humans ; Immunoblotting ; Insecta ; Insects ; Kinesin ; Kinesin - metabolism ; Kinetics ; Life Sciences ; Luminescent Proteins ; Luminescent Proteins - metabolism ; Metaphase ; Microscopy, Fluorescence ; Microtubules ; Microtubules - metabolism ; Mitosis ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Phosphoproteins ; Phosphoproteins - metabolism ; Phosphoproteins - physiology ; Phosphorylation ; Protein Structure, Tertiary ; Recombinant Fusion Proteins ; Recombinant Fusion Proteins - metabolism ; Recombinant Proteins ; Recombinant Proteins - metabolism ; RNA Interference ; RNA, Small Interfering ; RNA, Small Interfering - metabolism ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Time Factors ; Tissue Distribution ; Transfection
É parte de: The Journal of biological chemistry, 2003-07, Vol.278 (30), p.27844-27852
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
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A. A. and J-M. S. contributed equally to this work
Descrição: The human M phase phosphoprotein 1 (MPP1), previously identified through a screening of a subset of proteins specifically phosphorylated at the G2/M transition (Matsumoto-Taniura, N., Pirollet, F., Monroe, R., Gerace, L., and Westendorf, J. M. (1996) Mol. Biol. Cell 7, 1455–1469), is characterized as a plus-end-directed kinesin-related protein. Recombinant MPP1 exhibits in vitro microtubule-binding and microtubule-bundling properties as well as microtubule-stimulated ATPase activity. In gliding experiments using polarity-marked microtubules, MPP1 is a slow molecular motor that moves toward the microtubule plus-end at a 0.07 μm/s speed. In cycling cells, MPP1 localizes mainly to the nuclei in interphase. During mitosis, MPP1 is diffuse throughout the cytoplasm in metaphase and subsequently localizes to the midzone to further concentrate on the midbody. MPP1 suppression by RNA interference induces failure of cell division late in cytokinesis. We conclude that MPP1 is a new mitotic molecular motor required for completion of cytokinesis.
Editor: United States: Elsevier Inc
Idioma: Inglês

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M Phase Phosphoprotein 1 Is a Human Plus-end-directed Kinesin-related Protein Required for Cytokinesis

Abaza, Aouatef ; Soleilhac, Jean-Marc ; Westendorf, Joanne ; Piel, Matthieu ; Crevel, Isabelle ; Roux, Aurélien ; Pirollet, Fabienne

United States: Elsevier Inc

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