Idioma:

Metabolomics Identifies Multiple Candidate Biomarkers to Diagnose and Stage Human African Trypanosomiasis

Vincent, Isabel M ; Daly, Rónán ; Courtioux, Bertrand ; Cattanach, Amy M ; Biéler, Sylvain ; Ndung'u, Joseph M ; Bisser, Sylvie ; Barrett, Michael P Masiga, Daniel K.

PLoS neglected tropical diseases, 2016-12, Vol.10 (12), p.e0005140-e0005140 [Periódico revisado por pares]

United States: Public Library of Science

Texto completo disponível

Citações Citado por

Enviar para

Título:
Metabolomics Identifies Multiple Candidate Biomarkers to Diagnose and Stage Human African Trypanosomiasis
Autor: Vincent, Isabel M ; Daly, Rónán ; Courtioux, Bertrand ; Cattanach, Amy M ; Biéler, Sylvain ; Ndung'u, Joseph M ; Bisser, Sylvie ; Barrett, Michael P
Masiga, Daniel K.
Assuntos: 5-Hydroxytryptophan - blood ; 5-Hydroxytryptophan - cerebrospinal fluid ; 5-Hydroxytryptophan - isolation & purification ; 5-Hydroxytryptophan - urine ; Adolescent ; Adult ; Biological markers ; Biology and Life Sciences ; Biomarkers ; Biomarkers - analysis ; Biomarkers - blood ; Biomarkers - cerebrospinal fluid ; Biomarkers - urine ; Blood ; Blood-Brain Barrier ; Care and treatment ; Cerebrospinal fluid ; Colleges & universities ; Diagnosis ; Drugs ; Female ; Funding ; Humans ; Infections ; Inflammation ; Life Sciences ; Male ; Medicine and Health Sciences ; Metabolism ; Metabolites ; Metabolomics - methods ; Neopterin - blood ; Neopterin - cerebrospinal fluid ; Neopterin - isolation & purification ; Neopterin - urine ; Parasites ; Parasitology ; Plasma ; Principal components analysis ; Regression Analysis ; Santé publique et épidémiologie ; Sensitivity and Specificity ; Tropical diseases ; Trypanosoma brucei gambiense - metabolism ; Trypanosomiasis ; Trypanosomiasis, African - diagnosis ; Trypanosomiasis, African - parasitology ; Urine ; Young Adult
É parte de: PLoS neglected tropical diseases, 2016-12, Vol.10 (12), p.e0005140-e0005140
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMCID: PMC5152828
Conceptualization: MPB SBié JMN.Data curation: SBié SBis JMN RD.Formal analysis: IMV RD.Funding acquisition: MPB JMN.Investigation: IMV AMC BC SBis.Methodology: IMV RD.Project administration: MPB SBis.Resources: BC SBis.Software: RD.Supervision: MPB SBis.Visualization: IMV RD SBis.Writing – original draft: IMV MPB SBié.Writing – review & editing: RD BC AMC SBié JMN.
The authors have declared that no competing interests exist.
Descrição: Treatment for human African trypanosomiasis is dependent on the species of trypanosome causing the disease and the stage of the disease (stage 1 defined by parasites being present in blood and lymphatics whilst for stage 2, parasites are found beyond the blood-brain barrier in the cerebrospinal fluid (CSF)). Currently, staging relies upon detecting the very low number of parasites or elevated white blood cell numbers in CSF. Improved staging is desirable, as is the elimination of the need for lumbar puncture. Here we use metabolomics to probe samples of CSF, plasma and urine from 40 Angolan patients infected with Trypanosoma brucei gambiense, at different disease stages. Urine samples provided no robust markers indicative of infection or stage of infection due to inherent variability in urine concentrations. Biomarkers in CSF were able to distinguish patients at stage 1 or advanced stage 2 with absolute specificity. Eleven metabolites clearly distinguished the stage in most patients and two of these (neopterin and 5-hydroxytryptophan) showed 100% specificity and sensitivity between our stage 1 and advanced stage 2 samples. Neopterin is an inflammatory biomarker previously shown in CSF of stage 2 but not stage 1 patients. 5-hydroxytryptophan is an important metabolite in the serotonin synthetic pathway, the key pathway in determining somnolence, thus offering a possible link to the eponymous symptoms of "sleeping sickness". Plasma also yielded several biomarkers clearly indicative of the presence (87% sensitivity and 95% specificity) and stage of disease (92% sensitivity and 81% specificity). A logistic regression model including these metabolites showed clear separation of patients being either at stage 1 or advanced stage 2 or indeed diseased (both stages) versus control.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Metabolomics Identifies Multiple Candidate Biomarkers to Diagnose and Stage Human African Trypanosomiasis

Vincent, Isabel M ; Daly, Rónán ; Courtioux, Bertrand ; Cattanach, Amy M ; Biéler, Sylvain ; Ndung'u, Joseph M ; Bisser, Sylvie ; Barrett, Michael P Masiga, Daniel K.

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.