Idioma:

Distinct Gene Set Enrichment Profiles in Eosinophilic and Non-Eosinophilic Chronic Rhinosinusitis with Nasal Polyps by Bulk RNA Barcoding and Sequencing

Ishino, Takashi ; Takeno, Sachio ; Takemoto, Kota ; Yamato, Kensuke ; Oda, Takashi ; Nishida, Manabu ; Horibe, Yuichiro ; Chikuie, Nobuyuki ; Kono, Takashi ; Taruya, Takayuki ; Hamamoto, Takao ; Ueda, Tsutomu

International journal of molecular sciences, 2022-05, Vol.23 (10), p.5653 [Periódico revisado por pares]

Switzerland: MDPI AG

Texto completo disponível

Citações Citado por

Enviar para

Título:
Distinct Gene Set Enrichment Profiles in Eosinophilic and Non-Eosinophilic Chronic Rhinosinusitis with Nasal Polyps by Bulk RNA Barcoding and Sequencing
Autor: Ishino, Takashi ; Takeno, Sachio ; Takemoto, Kota ; Yamato, Kensuke ; Oda, Takashi ; Nishida, Manabu ; Horibe, Yuichiro ; Chikuie, Nobuyuki ; Kono, Takashi ; Taruya, Takayuki ; Hamamoto, Takao ; Ueda, Tsutomu
Assuntos: Annotations ; Aspirin ; Asthma ; Bar codes ; BRB-seq ; Chronic Disease ; chronic rhinosinusitis (CRS) ; Cluster analysis ; Comorbidity ; CRS endotypes ; Cytokines ; Encyclopedias ; eosinophils ; Epidemiology ; Gene expression ; Genes ; Genomes ; Humans ; Inflammation ; Inflammatory diseases ; Leukocytes (eosinophilic) ; Metabolism ; nasal polyps ; Nasal Polyps - complications ; Nasal Polyps - genetics ; Nasal Polyps - metabolism ; paranasal sinuses ; Pathogenesis ; Phenotypes ; Polyps ; Rhinitis ; Rhinitis - complications ; Rhinitis - genetics ; Rhinosinusitis ; RNA ; Sinusitis ; Sinusitis - complications ; Sinusitis - genetics ; Smell ; Transcriptomes ; Transcriptomics
É parte de: International journal of molecular sciences, 2022-05, Vol.23 (10), p.5653
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammatory disease with a high symptom burden, including nasal congestion and smell disorders. This study performed a detailed transcriptomic analysis in CRSwNP classified as eosinophilic CRS (ECRS), nonECRS according to the Japanese Epidemiological Survey of Refractory Eosinophilic Chronic Rhinosinusitis (JESREC) criteria, and a group of ECRS with comorbid aspirin intolerant asthma (Asp). Gene expression profiles of nasal polyps and the uncinate process in CRSwNP patients and normal subjects (controls) were generated by bulk RNA barcoding and sequencing (BRB-seq). A differentially expressed genes (DEGs) analysis was performed using DESeq2 software in iDEP to clarify any relationship between gene expression and disease backgrounds. A total of 3004 genes were identified by DEGs analysis to be associated with ECRS vs control, nonECRS vs control, and Asp vs control. A pathway analysis showed distinct profiles between the groups. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) showed distinct phenotype-specific pathways of expressed genes. In the specific pathway of "cytokine-cytokine receptor interaction", the differentially expressed genes were widely distributed. This study indicates that transcriptome analysis using BRB-seq may be a valuable tool to explore the pathogenesis of type 2 inflammation in CRSwNP.
Editor: Switzerland: MDPI AG
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 4 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Distinct Gene Set Enrichment Profiles in Eosinophilic and Non-Eosinophilic Chronic Rhinosinusitis with Nasal Polyps by Bulk RNA Barcoding and Sequencing

Ishino, Takashi ; Takeno, Sachio ; Takemoto, Kota ; Yamato, Kensuke ; Oda, Takashi ; Nishida, Manabu ; Horibe, Yuichiro ; Chikuie, Nobuyuki ; Kono, Takashi ; Taruya, Takayuki ; Hamamoto, Takao ; Ueda, Tsutomu

Switzerland: MDPI AG

Buscando em bases de dados remotas. Favor aguardar.