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NF‐YB1‐YC12‐bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)

Bello, Babatunde Kazeem ; Hou, Yuxuan ; Zhao, Juan ; Jiao, Guiai ; Wu, Yawen ; Li, Zhiyong ; Wang, Yifeng ; Tong, Xiaohong ; Wang, Wei ; Yuan, Wenya ; Wei, Xiangjin ; Zhang, Jian

Plant biotechnology journal, 2019-07, Vol.17 (7), p.1222-1235 [Periódico revisado por pares]

England: John Wiley & Sons, Inc

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Título:
NF‐YB1‐YC12‐bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)
Autor: Bello, Babatunde Kazeem ; Hou, Yuxuan ; Zhao, Juan ; Jiao, Guiai ; Wu, Yawen ; Li, Zhiyong ; Wang, Yifeng ; Tong, Xiaohong ; Wang, Wei ; Yuan, Wenya ; Wei, Xiangjin ; Zhang, Jian
Assuntos: Analysis ; Basic Helix-Loop-Helix Transcription Factors - metabolism ; DNA binding proteins ; Edible Grain ; Gene Expression Regulation, Plant ; Genetic transcription ; grain quality ; G‐box ; heterotrimer complex ; nuclear factor Y ; Oryza - enzymology ; Oryza - genetics ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Quality control ; Quality management ; Rice ; rice (Oryza sativa L.) ; RNA ; Seeds ; Starch Synthase - genetics ; Starch Synthase - metabolism ; Wx gene
É parte de: Plant biotechnology journal, 2019-07, Vol.17 (7), p.1222-1235
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
These authors contributed equally to this work.
Descrição: Summary Identification of seed development regulatory genes is the key for the genetic improvement in rice grain quality. NF‐Ys are the important transcription factors, but their roles in rice grain quality control and the underlying molecular mechanism remain largely unknown. Here, we report the functional characterization a rice NF‐Y heterotrimer complex NF‐YB1‐YC12‐bHLH144, which is formed by the binding of NF‐YB1 to NF‐YC12 and then bHLH144 in a sequential order. Knock‐out of each of the complex genes resulted in alteration of grain qualities in all the mutants as well as reduced grain size in crnf‐yb1 and crnf‐yc12. RNA‐seq analysis identified 1496 genes that were commonly regulated by NF‐YB1 and NF‐YC12, including the key granule‐bound starch synthase gene Wx. NF‐YC12 and bHLH144 maintain NF‐YB1 stability from the degradation mediated by ubiquitin/26S proteasome, while NF‐YB1 directly binds to the ‘G‐box’ domain of Wx promoter and activates Wx transcription, hence to regulate rice grain quality. Finally, we revealed a novel grain quality regulatory pathway controlled by NF‐YB1‐YC12‐bHLH144 complex, which has great potential for rice genetic improvement.
Editor: England: John Wiley & Sons, Inc
Idioma: Inglês

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NF‐YB1‐YC12‐bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)

Bello, Babatunde Kazeem ; Hou, Yuxuan ; Zhao, Juan ; Jiao, Guiai ; Wu, Yawen ; Li, Zhiyong ; Wang, Yifeng ; Tong, Xiaohong ; Wang, Wei ; Yuan, Wenya ; Wei, Xiangjin ; Zhang, Jian

England: John Wiley & Sons, Inc

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