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Quantitative Neuropathology by High Resolution Magic Angle Spinning Proton Magnetic Resonance Spectroscopy

Cheng, L. L. ; Ma, M. J. ; Becerra, L. ; Ptak, T. ; Tracey, I. ; Lackner, A. ; González, R. G.

Proceedings of the National Academy of Sciences - PNAS, 1997-06, Vol.94 (12), p.6408-6413 [Periódico revisado por pares]

United States: National Academy of Sciences of the United States of America

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Título:
Quantitative Neuropathology by High Resolution Magic Angle Spinning Proton Magnetic Resonance Spectroscopy
Autor: Cheng, L. L. ; Ma, M. J. ; Becerra, L. ; Ptak, T. ; Tracey, I. ; Lackner, A. ; González, R. G.
Assuntos: Amino Acids - analysis ; Animals ; Aspartic Acid - analogs & derivatives ; Aspartic Acid - analysis ; Biological Sciences ; Biomarkers ; Brain ; Brain - pathology ; Cerebral Cortex - pathology ; Dementia - pathology ; Haplorhini ; High resolution ; Humans ; Magnetic Resonance Spectroscopy - methods ; Medical imaging ; Medical research ; Metabolic brain diseases ; Nerve tissue ; Neurology ; Neurons ; Neurons - pathology ; Organ Specificity ; Pathology - methods ; Pick disease of the brain ; Protons ; Reproducibility of Results ; Spectral resolution ; Tissue samples ; Visual cortex
É parte de: Proceedings of the National Academy of Sciences - PNAS, 1997-06, Vol.94 (12), p.6408-6413
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Alfred G. Redfield, Brandeis University, Lexington, MA
To whom reprint requests should be addressed at: Massachusetts General Hospital NMR Center, 149 13th Street, Charlestown, MA 02129. e-mail: gil@nmr.mgh.harvard.edu.
Descrição: We describe a method that directly relates tissue neuropathological analysis to medical imaging. Presently, only indirect and often tenuous relationships are made between imaging (such as MRI or x-ray computed tomography) and neuropathology. We present a biochemistry-based, quantitative neuropathological method that can help to precisely quantify information provided by in vivo proton magnetic resonance spectroscopy (1HMRS), an emerging medical imaging technique. This method, high resolution magic angle spinning (HRMAS) 1HMRS, is rapid and requires only small amounts of unprocessed samples. Unlike chemical extraction or other forms of tissue processing, this method analyzes tissue directly, thus minimizing artifacts. We demonstrate the utility of this method by assessing neuronal damage using multiple tissue samples from differently affected brain regions in a case of Pick disease, a human neurodegenerative disorder. Among different regions, we found an excellent correlation between neuronal loss shown by traditional neurohistopathology and decrease of the neuronal marker N-acetylaspartate measured by HRMAS 1HMRS. This result demonstrates for the first time, to our knowledge, a direct, quantitative link between a decrease in N-acetylaspartate and neuronal loss in a human neurodegenerative disease. As a quantitative method, HRMAS 1HMRS has potential applications in experimental and clinical neuropathologic investigations. It should also provide a rational basis for the interpretation of in vivo 1HMRS studies of human neurological disorders.
Editor: United States: National Academy of Sciences of the United States of America
Idioma: Inglês

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Quantitative Neuropathology by High Resolution Magic Angle Spinning Proton Magnetic Resonance Spectroscopy

Cheng, L. L. ; Ma, M. J. ; Becerra, L. ; Ptak, T. ; Tracey, I. ; Lackner, A. ; González, R. G.

United States: National Academy of Sciences of the United States of America

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