Idioma:

The housekeeping promoter from the mouse CpG island HTF9 contains multiple protein-binding elements that are functionally redundant

SOMMA, M. P ; PISANO, C ; LAVIA, P

Nucleic acids research, 1991-06, Vol.19 (11), p.2817-2824 [Periódico revisado por pares]

Oxford: Oxford University Press

Texto completo disponível

Citações Citado por

Enviar para

Título:
The housekeeping promoter from the mouse CpG island HTF9 contains multiple protein-binding elements that are functionally redundant
Autor: SOMMA, M. P ; PISANO, C ; LAVIA, P
Assuntos: Animals ; Base Sequence ; Binding, Competitive ; Biological and medical sciences ; Blotting, Southern ; Blotting, Western ; Chloramphenicol O-Acetyltransferase - genetics ; Chromosome Deletion ; Dinucleoside Phosphates - genetics ; DNA Fingerprinting ; DNA-Binding Proteins - metabolism ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Promoter Regions, Genetic ; RNA - genetics ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing
É parte de: Nucleic acids research, 1991-06, Vol.19 (11), p.2817-2824
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Descrição: The mouse CpG-rich island HTF9 harbours the divergent RNA initiation sites shared by two genes that are both expressed in a housekeeping fashion. In this work we have analyzed the architecture of the HTF9 promoter. Gel shift assays were first employed to locate nuclear factor-binding sites within HTF9. Multiple protein-binding sites were identified across a 500 bp-long region, two of which appear to interact with novel factors. Deletion analysis was used to determine the requirements for the different sites in transient expression of a CAT reporter gene. Although multiple elements contributed to the overall promoter strength in each orientation, extensive deletions failed to affect the basal level of transcription from HTF9 in either direction. Thus, only a subset of elements is necessary to activate transcription from HTF9. Functional redundancy may be a general feature of housekeeping CpG-rich promoters.
Editor: Oxford: Oxford University Press
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

The housekeeping promoter from the mouse CpG island HTF9 contains multiple protein-binding elements that are functionally redundant

SOMMA, M. P ; PISANO, C ; LAVIA, P

Oxford: Oxford University Press

Buscando em bases de dados remotas. Favor aguardar.