The catalytic pathway of horseradish peroxidase at high resolution

• Autor: Hajdu, Janos ; Berglund, Gunnar I ; Carlsson, Gunilla H ; Smith, Andrew T ; Szöke, Hanna ; Henriksen, Anette
• Assuntos: Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Catalysis - radiation effects ; Catalysts ; Crystallization ; Crystallography, X-Ray ; Crystals ; Enzymes ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Horseradish Peroxidase - chemistry ; Horseradish Peroxidase - metabolism ; Iron - chemistry ; Iron - metabolism ; Metabolism ; Models, Molecular ; Molecules ; Oxidation-Reduction - radiation effects ; Oxidoreductases ; Oxygen ; Oxygen - metabolism ; Plant physiology and development ; Protein Conformation - radiation effects ; X-Rays
• É parte de: Nature (London), 2002-05, Vol.417 (6887), p.463-468
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• Descrição: A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.
  
• Editor: London: Nature Publishing
  
• Idioma: Inglês