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Maned wolf (Chrysocyon brachyurus) urinary volatile organic compounds

0000-0001-5045-6725 ; Songsasen, Nucharin ; Huff, Thomas B ; Freeman, Elizabeth W

figshare 2021

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  • Título:
    Maned wolf (Chrysocyon brachyurus) urinary volatile organic compounds
  • Autor: 0000-0001-5045-6725 ; Songsasen, Nucharin ; Huff, Thomas B ; Freeman, Elizabeth W
  • Assuntos: Animal Developmental and Reproductive Biology ; FOS: Biological sciences ; Zoology
  • Descrição: These data are raw peak areas for urinary VOCs in the urine samples of 13 maned wolves (Chrysocyon brachyurus) collected weekly for 6 months over one breeding season. Information about each individual wolf is provided in the table below: CallName SB# Age Sex Institution Housed with Pairing Status Breeding Information Bonita 3231 3 F Beardsley SB#3232 Unpaired NA Same sex sibling pair Chiquita 3232 3 F Beardsley SB#3231 Unpaired NA Same sex sibling pair Yellow 2845 8 F Fossil Rim * Unpaired None seen Mac 3206 4 M Fossil Rim SB#3207 (sister) Unpaired NA Female spayed Cheech 3192 3 M Philadelphia SB#3195 Unpaired NA Same sex sibling pair Chong 3195 3 M Philadelphia SB#3192 Unpaired NA Same sex sibling pair Flint 2810 8 M SCBI Single Unpaired NA Single Blue 2844 8 M SCBI SB#3184 Paired None seen Uno 3184 4 F SCBI SB#2844 Paired None seen Zonka 2660 10 M WOCC SB#2945 Paired Estimated breeding date = Oct 8, 2014 Kim 2945 8 F WOCC SB#2660 Paired Estimated breeding date = Oct 8, 2014 Elisa 2536 11 F Buffalo SB#3014 N/A N/A Male neutered Kev 3014 7 NeutM Buffalo SB#2536 N/A N/A Male neutered Raw data from GC-MS in MassHunter’s .D format were converted into .mzXML format for data processing steps using ProteoWizard’s MSConvert tool. Files from replicates of each sample were processed together through the xcms R package using R ver 3.6.1. The xcms package contains functions to identify ion peaks, group the same ion peaks across replicates, correct retention time drift using the Obiwarp algorithm, and then use the corrected retention times to regroup ion peaks across replicates. Next, missing ion peaks were filled in. Then a deconvolution algorithm in the camera R package assigned ion peaks into compounds. Ions with the same peak shapes at the same retention times were attributed to the same group to create a compound made up of component ions. This open-source data analysis pipeline is robust, free of cost, and easily accessible, allowing analysis of hundreds or thousands of samples containing millions of ion peaks on a personal computer. Thus, this same data analysis pipeline could easily extend to other species or areas of VOC research. Ion peak areas were then averaged across the three replicates of each sample, creating one mean value per urine sample for each ion. Within each compound group, only the peak area for the quantitative ion (ion with the largest average area) was retained. This value is representative of the abundance of the VOC in that sample. These quantitative ion peak areas are the data that are shared.
  • Editor: figshare
  • Data de criação/publicação: 2021
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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