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Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination

Blow, J. Julian ; Li, Anatoliy

Nature cell biology, 2004-03, Vol.6 (3), p.260-267 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination
Autor: Blow, J. Julian ; Li, Anatoliy
Assuntos: Anaphase-Promoting Complex-Cyclosome ; Animals ; Cell cycle ; Cell Cycle Proteins - metabolism ; Cell Extracts - chemistry ; Cell-Free System - chemistry ; Cell-Free System - metabolism ; Chromosomes - physiology ; Cyclin-Dependent Kinases - metabolism ; DNA replication ; DNA Replication - physiology ; Female ; G1 Phase - physiology ; Geminin ; Genetic aspects ; Male ; Metaphase - physiology ; Mitosis - physiology ; Ovum - chemistry ; Peptide Hydrolases - metabolism ; Physiological aspects ; Spermatozoa - chemistry ; Ubiquitin - metabolism ; Ubiquitin-proteasome system ; Ubiquitin-Protein Ligase Complexes - metabolism ; Xenopus ; Xenopus laevis ; Xenopus Proteins
É parte de: Nature cell biology, 2004-03, Vol.6 (3), p.260-267
Notas: ObjectType-Article-2
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Descrição: In late mitosis and G1, a complex of the essential initiation proteins Mcm2-7 are assembled onto replication origins to 'license' them for initiation. At other times licensing is inhibited by cyclin-dependent kinases (CDKs) and geminin, thus ensuring that origins fire only once per cell cycle. Here we show that, paradoxically, CDKs are also required to inactivate geminin and activate the licensing system. On exit from metaphase in Xenopus laevis egg extracts, CDK-dependent activation of the anaphase-promoting complex (APC/C) results in the transient polyubiquitination of geminin. This ubiquitination triggers geminin inactivation without requiring ubiquitin-dependent proteolysis, and is essential for replication origins to become licensed. This reveals an unexpected role for CDKs and ubiquitination in activating chromosomal DNAreplication.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination

Blow, J. Julian ; Li, Anatoliy

England: Nature Publishing Group

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