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Structure and chromosomal location of the human gene encoding cartilage matrix protein

Jenkins, R N ; Osborne-Lawrence, S L ; Sinclair, A K ; Eddy, R L ; Byers, M G ; Shows, T B ; Duby, A D

The Journal of biological chemistry, 1990-11, Vol.265 (32), p.19624-19631 [Periódico revisado por pares]

Bethesda, MD: Elsevier Inc

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Título:
Structure and chromosomal location of the human gene encoding cartilage matrix protein
Autor: Jenkins, R N ; Osborne-Lawrence, S L ; Sinclair, A K ; Eddy, R L ; Byers, M G ; Shows, T B ; Duby, A D
Assuntos: Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Cartilage Oligomeric Matrix Protein ; Chickens ; Chromosome Mapping ; Cloning, Molecular ; DNA Probes ; Exons ; Extracellular Matrix Proteins ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Glycoproteins - genetics ; Glycosylation ; Humans ; Introns ; Matrilin Proteins ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Polymorphism, Genetic ; Promoter Regions, Genetic - genetics ; Protein Biosynthesis ; Restriction Mapping ; RNA Splicing ; Sequence Homology, Nucleic Acid
É parte de: The Journal of biological chemistry, 1990-11, Vol.265 (32), p.19624-19631
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Cartilage matrix protein (CMP) is a major component of the extracellular matrix of nonarticular cartilage. The structure and chromosomal location of the human gene encoding CMP was determined by molecular cloning analysis. We used a partial chicken CMP cDNA probe to isolate three overlapping human genomic clones. From one of these clones, a probe containing 2 human CMP exons was isolated and used to map the gene to chromosome 1p35 and to screen a human retina cDNA library. Two overlapping cDNA clones were isolated. The predicted protein sequence of 496 amino acids includes a 22-residue signal peptide and a 474-residue mature protein of Mr 51,344. The human CMP gene and polypeptide are strikingly similar to the chicken CMP gene and polypeptide. Human CMP is 79% identical to chicken CMP and contains two homologous domains separated by an epidermal growth factor-like domain. One potential N-glycosylation site is conserved between the two species. The human CMP gene spans 12 kilobase pairs with 8 exons and 7 introns which are similar in size to those of the chicken CMP gene. Both RNA splice junctions of intron G in the human and chicken CMP genes are nonconforming to the consensus splice sequences. This suggests that the CMP gene utilizes a new RNA splicing mechanism.
Editor: Bethesda, MD: Elsevier Inc
Idioma: Inglês

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Structure and chromosomal location of the human gene encoding cartilage matrix protein

Jenkins, R N ; Osborne-Lawrence, S L ; Sinclair, A K ; Eddy, R L ; Byers, M G ; Shows, T B ; Duby, A D

Bethesda, MD: Elsevier Inc

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