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Ovocalyxin-36 is a pattern recognition protein in chicken eggshell membranes

Cordeiro, Cristianne M M ; Esmaili, Hamed ; Ansah, George ; Hincke, Maxwell T Uversky, Vladimir N.

PloS one, 2013-12, Vol.8 (12), p.e84112-e84112 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Ovocalyxin-36 is a pattern recognition protein in chicken eggshell membranes
Autor: Cordeiro, Cristianne M M ; Esmaili, Hamed ; Ansah, George ; Hincke, Maxwell T
Uversky, Vladimir N.
Assuntos: Acute-Phase Proteins - genetics ; Acute-Phase Proteins - isolation & purification ; Acute-Phase Proteins - metabolism ; Amino acid sequence ; Animals ; Anion exchange ; Anion exchanging ; Anti-Infective Agents - isolation & purification ; Anti-Infective Agents - metabolism ; Antimicrobial Cationic Peptides - genetics ; Antimicrobial Cationic Peptides - isolation & purification ; Antimicrobial Cationic Peptides - metabolism ; Bacteria ; Bacteria - pathogenicity ; Bacterial Infections - metabolism ; Bacterial Infections - microbiology ; Bacterial Infections - prevention & control ; Biology ; Blood Proteins - genetics ; Blood Proteins - isolation & purification ; Blood Proteins - metabolism ; Blotting, Western ; BPI protein ; Breeding success ; Calcification ; Carrier Proteins - genetics ; Carrier Proteins - isolation & purification ; Carrier Proteins - metabolism ; Cation exchanging ; Cell Membrane - metabolism ; Chickens ; Chromatography, Liquid ; Chromosomes ; E coli ; Egg Proteins - genetics ; Egg Proteins - isolation & purification ; Egg Proteins - metabolism ; Egg shells ; Eggs ; Enzyme-Linked Immunosorbent Assay ; Epithelium ; Escherichia coli ; Fabrication ; Food safety ; Homology ; Lipopolysaccharide-binding protein ; Lipopolysaccharides ; Lipopolysaccharides - metabolism ; Lipoteichoic acid ; Lungs ; Mammals ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - isolation & purification ; Membrane Glycoproteins - metabolism ; Membrane permeability ; Membrane proteins ; Membranes ; Mitogens ; Molecular weight ; Organic acids ; Palate ; Pattern recognition ; Permeability ; Phosphatase ; Polymorphism, Single Nucleotide - genetics ; Proline ; Protein binding ; Protein purification ; Proteins ; Reproduction ; Sepsis ; Serine ; Single nucleotide polymorphisms ; Single-nucleotide polymorphism ; Staphylococcus aureus ; Tandem Mass Spectrometry ; Teichoic Acids - metabolism
É parte de: PloS one, 2013-12, Vol.8 (12), p.e84112-e84112
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: GA is an employee of Hendrix Genetics. The material contribution of Hendrix Genetics to the research was the provision of blood samples and eggs from specific birds in their flocks. There are no further patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.
Conceived and designed the experiments: CMMC GA MTH. Performed the experiments: CMMC HE. Analyzed the data: CMMC HE GA MTH. Contributed reagents/materials/analysis tools: MTH GA. Wrote the paper: CMMC HE GA MTH.
Descrição: The avian eggshell membranes are essential elements in the fabrication of the calcified shell as a defense against bacterial penetration. Ovocalyxin-36 (OCX-36) is an abundant avian eggshell membrane protein, which shares protein sequence homology to bactericidal permeability-increasing protein (BPI), lipopolysaccharide-binding protein (LBP) and palate, lung and nasal epithelium clone (PLUNC) proteins. We have developed an efficient method to extract OCX-36 from chicken eggshell membranes for purification with cation and anion exchange chromatographies. Purified OCX-36 protein exhibited lipopolysaccharide (LPS) binding activity and bound lipopolysaccharide (LPS) from Escherichia coli O111:B4 in a dose-dependent manner. OCX-36 showed inhibitory activity against growth of Staphylococcus aureus ATCC 6538. OCX-36 single nucleotide polymorphisms (SNPs) were verified at cDNA 211 position and the corresponding proteins proline-71 (Pro-71) or serine-71 (Ser-71) were purified from eggs collected from genotyped hens. A significant difference between Pro-71 and Ser-71 OCX-36 for S. aureus lipoteichoic acid (LTA) binding activity was detected. The current study is a starting point to understand the innate immune role that OCX-36 may play in protection against bacterial invasion of both embryonated eggs (relevant to avian reproductive success) and unfertilized table eggs (relevant to food safety).
Editor: United States: Public Library of Science
Idioma: Inglês

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Ovocalyxin-36 is a pattern recognition protein in chicken eggshell membranes

Cordeiro, Cristianne M M ; Esmaili, Hamed ; Ansah, George ; Hincke, Maxwell T Uversky, Vladimir N.

United States: Public Library of Science

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