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Hydroquinone diminishes levels of anaphylatic antibodies in allergic rats

S M D Macedo S C M Vaz; Wothan Tavares de Lima; Sandra Helena Poliselli Farsky; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)

Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006

São Paulo 2006

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  • Título:
    Hydroquinone diminishes levels of anaphylatic antibodies in allergic rats
  • Autor: S M D Macedo
  • S C M Vaz; Wothan Tavares de Lima; Sandra Helena Poliselli Farsky; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)
  • Assuntos: FARMACOLOGIA
  • É parte de: Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006
  • Notas: Disponível em CD-ROM
  • Descrição: Introduction and Objectives: Human exposure to Hydroquinone (HQ) occurs in environmental and occupational conditions. We previously showed that HQ exposure impairs the pulmonary allergic inflammation elicited by ovalbumin (OA) in rats (Toxicol. Sci. Submitted, 2006). Here we investigated possible mechanisms involved this impaired response. Methods and Results: Male Wistar rats were intraperitoneally exposed to HQ (50mg/Kg) once a day, for 22 days. Control animals received vehicle. On day 10, animals were intraperitoneally sensitised with OA. Thirteen days after sensitisation and 24 h after the last injection of HQ or vehicle, spleen lymphocytes were collected to proliferative and flow citometry assay and blood was collected from abdominal aorta to obtain serum used in passive cutaneous anaphylactic (PCA) test. Other set of animals was challenged by topical application into mesentery to evaluate the mast cell degranulation by intravital microscopy. HQ exposure determined lower levels of circulating OAanaphylactic antibodies (Vehicle: 8 ± 0.3; HQ: 2.8 ± 1.1 title of PCA, n=4, p<0.001) and reduced mast cell degranulation (colour intensity of toluidin dye), showing that HQ exposure impairs the anaphylactic antibodies production and the consequent mast cell degranulation. This hypothesis was supported by equivalent mast cell degranulation after 48/80 compound topical application. Spleen lymphocytes proliferation in response to OA was not
    altered (Vehicle: 1186.5 ± 71.20; HQ: 1585.1 ± 88,21 [3H]-TdR incorporation/cpm, n=3), but the B lymphocyte clone in differentiation was significantly lower in HQ exposed rats (Vehicle: 135.1 ± 9.1; HQ: 115.2 ± 7.3 intensity of fluorescence/arbitrary unit, n=3, p<0.05). Conclusion: Results indicated that extended exposure to HQ impairs "in vivo" humoral immunological response, which may contribute to reduced mast cell degranulation and local inflammation.
  • Editor: São Paulo
  • Data de criação/publicação: 2006
  • Formato: res. IR.033.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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