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Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics

Ferro, Federico ; Spelat, Renza ; D'Aurizio, Federica ; Puppato, Elisa ; Pandolfi, Maura ; Beltrami, Antonio Paolo ; Cesselli, Daniela ; Falini, Giuseppe ; Beltrami, Carlo Alberto ; Curcio, Francesco Cooney, Austin John

PloS one, 2012-07, Vol.7 (7), p.e41774-e41774 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics
Autor: Ferro, Federico ; Spelat, Renza ; D'Aurizio, Federica ; Puppato, Elisa ; Pandolfi, Maura ; Beltrami, Antonio Paolo ; Cesselli, Daniela ; Falini, Giuseppe ; Beltrami, Carlo Alberto ; Curcio, Francesco
Cooney, Austin John
Assuntos: Adult Stem Cells - cytology ; Adult Stem Cells - metabolism ; Amino acids ; Antigens ; Biology ; Biomarkers ; Biomarkers - metabolism ; c-Myc protein ; Cancer ; Cancer research ; CD29 antigen ; Cell Differentiation ; Cell self-renewal ; Child ; Child, Preschool ; Cytoplasm ; Dental pulp ; Dental Pulp - cytology ; Differentiation (biology) ; Embryonic stem cells ; Embryos ; Fibroblasts ; Fourier transforms ; Gene Expression Regulation, Developmental ; Humans ; Kinases ; KLF4 protein ; Laboratories ; Localization ; Maintenance ; Medicine ; Myc protein ; Oct-4 protein ; Octamer Transcription Factor-3 - genetics ; Octamer Transcription Factor-3 - metabolism ; Oncology, Experimental ; Phosphorylation ; Pluripotency ; Post-translation ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; Protein Processing, Post-Translational ; Proteins ; Stem cell research ; Stem cells ; Teaching hospitals ; Transcription factors
É parte de: PloS one, 2012-07, Vol.7 (7), p.e41774-e41774
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: FF RS. Performed the experiments: FF RS FD EP MP GF. Analyzed the data: FF RS DC APB GF CAB FC. Wrote the paper: FF RS GF APB DC CAB FC.
Descrição: Although the role played by the core transcription factor network, which includes c-Myc, Klf4, Nanog, and Oct4, in the maintenance of embryonic stem cell (ES) pluripotency and in the reprogramming of adult cells is well established, its persistence and function in adult stem cells are still debated. To verify its persistence and clarify the role played by these molecules in adult stem cell function, we investigated the expression pattern of embryonic and adult stem cell markers in undifferentiated and fully differentiated dental pulp stem cells (DPSC). A particular attention was devoted to the expression pattern and intracellular localization of the stemness-associated isoform A of Oct4 (Oct4A). Our data demonstrate that: Oct4, Nanog, Klf4 and c-Myc are expressed in adult stem cells and, with the exception of c-Myc, they are significantly down-regulated following differentiation. Cell differentiation was also associated with a significant reduction in the fraction of DPSC expressing the stem cell markers CD10, CD29 and CD117. Moreover, a nuclear to cytoplasm shuttling of Oct4A was identified in differentiated cells, which was associated with Oct4A phosphorylation. The present study would highlight the importance of the post-translational modifications in DPSC stemness maintenance, by which stem cells balance self-renewal versus differentiation. Understanding and controlling these mechanisms may be of great importance for stemness maintenance and stem cells clinical use, as well as for cancer research.
Editor: United States: Public Library of Science
Idioma: Inglês

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Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics

Ferro, Federico ; Spelat, Renza ; D'Aurizio, Federica ; Puppato, Elisa ; Pandolfi, Maura ; Beltrami, Antonio Paolo ; Cesselli, Daniela ; Falini, Giuseppe ; Beltrami, Carlo Alberto ; Curcio, Francesco Cooney, Austin John

United States: Public Library of Science

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