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Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis

Berry, Jamie-Lee ; Gurung, Ishwori ; Anonsen, Jan Haug ; Spielman, Ingrid ; Harper, Elliot ; Hall, Alexander M.J. ; Goosens, Vivianne J. ; Raynaud, Claire ; Koomey, Michael ; Biais, Nicolas ; Matthews, Steve ; Pelicic, Vladimir

The Journal of biological chemistry, 2019-04, Vol.294 (17), p.6796-6808 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Autor: Berry, Jamie-Lee ; Gurung, Ishwori ; Anonsen, Jan Haug ; Spielman, Ingrid ; Harper, Elliot ; Hall, Alexander M.J. ; Goosens, Vivianne J. ; Raynaud, Claire ; Koomey, Michael ; Biais, Nicolas ; Matthews, Steve ; Pelicic, Vladimir
Assuntos: Bacteriology ; Biopolymers - metabolism ; Fimbriae Proteins - chemistry ; Fimbriae Proteins - metabolism ; Fimbriae, Bacterial - metabolism ; Gram-positive bacteria ; Life Sciences ; Methylation ; Microbiology ; Microbiology and Parasitology ; molecular motor ; pilin ; Protein Conformation ; protein structure ; Streptococcus - metabolism ; Streptococcus sanguinis ; twitching motility ; type IV filaments ; type IV pili ; virulence factor
É parte de: The Journal of biological chemistry, 2019-04, Vol.294 (17), p.6796-6808
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
NFR/249062
PMCID: PMC6497953
Recipient of a Ph.D. studentship from the MRC Centre for Molecular Bacteriology and Infection.
Both authors contributed equally to the results of this work.
Edited by Chris Whitfield
Descrição: Type IV pili (Tfp) are functionally versatile filaments, widespread in prokaryotes, that belong to a large class of filamentous nanomachines known as type IV filaments (Tff). Although Tfp have been extensively studied in several Gram-negative pathogens where they function as key virulence factors, many aspects of their biology remain poorly understood. Here, we performed a global biochemical and structural analysis of Tfp in a recently emerged Gram-positive model, Streptococcus sanguinis. In particular, we focused on the five pilins and pilin-like proteins involved in Tfp biology in S. sanguinis. We found that the two major pilins, PilE1 and PilE2, (i) follow widely conserved principles for processing by the prepilin peptidase PilD and for assembly into filaments; (ii) display only one of the post-translational modifications frequently found in pilins, i.e. a methylated N terminus; (iii) are found in the same heteropolymeric filaments; and (iv) are not functionally equivalent. The 3D structure of PilE1, solved by NMR, revealed a classical pilin-fold with a highly unusual flexible C terminus. Intriguingly, PilE1 more closely resembles pseudopilins forming shorter Tff than bona fide Tfp-forming major pilins, underlining the evolutionary relatedness among different Tff. Finally, we show that S. sanguinis Tfp contain a low abundance of three additional proteins processed by PilD, the minor pilins PilA, PilB, and PilC. These findings provide the first global biochemical and structural picture of a Gram-positive Tfp and have fundamental implications for our understanding of a widespread class of filamentous nanomachines.
Editor: United States: Elsevier Inc
Idioma: Inglês;Norueguês

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Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis

Berry, Jamie-Lee ; Gurung, Ishwori ; Anonsen, Jan Haug ; Spielman, Ingrid ; Harper, Elliot ; Hall, Alexander M.J. ; Goosens, Vivianne J. ; Raynaud, Claire ; Koomey, Michael ; Biais, Nicolas ; Matthews, Steve ; Pelicic, Vladimir

United States: Elsevier Inc

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