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Discovery of Leptospira spp. seroreactive peptides using ORFeome phage display

Ramli, Siti Roszilawati ; Moreira, Gustavo M S G ; Zantow, Jonas ; Goris, Marga G A ; Nguyen, Van Kinh ; Novoselova, Natalia ; Pessler, Frank ; Hust, Michael Coburn, Jenifer

PLoS neglected tropical diseases, 2019-01, Vol.13 (1), p.e0007131-e0007131 [Periódico revisado por pares]

United States: Public Library of Science

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Discovery of Leptospira spp. seroreactive peptides using ORFeome phage display
Autor: Ramli, Siti Roszilawati ; Moreira, Gustavo M S G ; Zantow, Jonas ; Goris, Marga G A ; Nguyen, Van Kinh ; Novoselova, Natalia ; Pessler, Frank ; Hust, Michael
Coburn, Jenifer
Assuntos: Animals ; Antibodies, Bacterial - blood ; Antibodies, Bacterial - immunology ; Antigens ; Bacterial Proteins - genetics ; Bacterial Proteins - immunology ; Bacterial Proteins - isolation & purification ; Biochemistry ; Bioinformatics ; Biology and Life Sciences ; Biomarkers ; Biotechnology ; Cell Surface Display Techniques ; Climate ; Dengue fever ; Detection ; Diagnosis ; Diagnostic systems ; ELISA ; Enzyme-linked immunosorbent assay ; Fatalities ; Genome, Bacterial - genetics ; Genomes ; Genomics ; Genomics - methods ; Glucose ; Gram-negative bacteria ; Gram-negative bacterial infections ; Hepatitis ; Humans ; Identification ; Immune response ; Immunogenicity ; Infections ; Infectious diseases ; Laboratories ; Leptospira ; Leptospira interrogans - genetics ; Leptospira interrogans - immunology ; Leptospira interrogans - isolation & purification ; Leptospirosis ; Leptospirosis - blood ; Leptospirosis - diagnosis ; Leptospirosis - microbiology ; Malaysia ; Medical research ; Medicine and Health Sciences ; Open Reading Frames ; Pathogenic microorganisms ; Pathogens ; Peptides ; Peptides - genetics ; Peptides - immunology ; Peptides - isolation & purification ; Phage display ; Phages ; Physiological aspects ; Proteins ; Reagents ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Recombinants ; Research and Analysis Methods ; Risk factors ; Salmonella ; Sensitivity ; Serology ; Specificity ; Strains ; Titration ; Tropical climate ; Tropical diseases ; Vaccines ; Zoonoses ; Zoonoses - blood ; Zoonoses - diagnosis ; Zoonoses - microbiology
É parte de: PLoS neglected tropical diseases, 2019-01, Vol.13 (1), p.e0007131-e0007131
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These authors are joint senior authors on this work.
The authors have declared that no competing interests exist.
Descrição: Leptospirosis is the most common zoonotic disease worldwide. The diagnostic performance of a serological test for human leptospirosis is mainly influenced by the antigen used in the test assay. An ideal serological test should cover all serovars of pathogenic leptospires with high sensitivity and specificity and use reagents that are relatively inexpensive to produce and can be used in tropical climates. Peptide-based tests fulfil at least the latter two requirements, and ORFeome phage display has been successfully used to identify immunogenic peptides from other pathogens. Two ORFeome phage display libraries of the entire Leptospira spp. genomes from five local strains isolated in Malaysia and seven WHO reference strains were constructed. Subsequently, 18 unique Leptospira peptides were identified in a screen using a pool of sera from patients with acute leptospirosis. Five of these were validated by titration ELISA using different pools of patient or control sera. The diagnostic performance of these five peptides was then assessed against 16 individual sera from patients with acute leptospirosis and 16 healthy donors and was compared to that of two recombinant reference proteins from L. interrogans. This analysis revealed two peptides (SIR16-D1 and SIR16-H1) from the local isolates with good accuracy for the detection of acute leptospirosis (area under the ROC curve: 0.86 and 0.78, respectively; sensitivity: 0.88 and 0.94; specificity: 0.81 and 0.69), which was close to that of the reference proteins LipL32 and Loa22 (area under the ROC curve: 0.91 and 0.80; sensitivity: 0.94 and 0.81; specificity: 0.75 and 0.75). This analysis lends further support for using ORFeome phage display to identify pathogen-associated immunogenic peptides, and it suggests that this technique holds promise for the development of peptide-based diagnostics for leptospirosis and, possibly, of vaccines against this pathogen.
Editor: United States: Public Library of Science
Idioma: Inglês

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Discovery of Leptospira spp. seroreactive peptides using ORFeome phage display

Ramli, Siti Roszilawati ; Moreira, Gustavo M S G ; Zantow, Jonas ; Goris, Marga G A ; Nguyen, Van Kinh ; Novoselova, Natalia ; Pessler, Frank ; Hust, Michael Coburn, Jenifer

United States: Public Library of Science

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