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SPR results: () Binding of NC to a 5

Fisher, Robert J. ; Fivash, Matthew J. ; Stephen, Andrew G. ; Hagan, Nathan A. ; Shenoy, Shilpa R. ; Medaglia, Maxine V. ; Smith, Lindsey R. ; Worthy, Karen M. ; Simpson, John T. ; Shoemaker, Robert ; McNitt, Karen L. ; Johnson, Donald G. ; Hixson, Catherine V. ; Gorelick, Robert J. ; Fabris, Daniele ; Henderson, Louis E. ; Rein, Alan

figshare 2011

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  • Título:
    SPR results: () Binding of NC to a 5
  • Autor: Fisher, Robert J. ; Fivash, Matthew J. ; Stephen, Andrew G. ; Hagan, Nathan A. ; Shenoy, Shilpa R. ; Medaglia, Maxine V. ; Smith, Lindsey R. ; Worthy, Karen M. ; Simpson, John T. ; Shoemaker, Robert ; McNitt, Karen L. ; Johnson, Donald G. ; Hixson, Catherine V. ; Gorelick, Robert J. ; Fabris, Daniele ; Henderson, Louis E. ; Rein, Alan
  • Assuntos: Uncategorized
  • Descrição: Copyright information:Taken from "Complex interactions of HIV-1 nucleocapsid protein with oligonucleotides"Nucleic Acids Research 2006;34(2):472-484.Published online 24 Jan 2006PMCID:PMC1351370.© The Author 2006. Published by Oxford University Press. All rights reserved4 RU surface of (TG) in 150 mM NaCl. Solutions of 1.7, 3.13, 6.25, 12.5, 25, 50, 100 and 200 nM NC were passed over the surface at 100 µl/min. The horizontal line represents the response expected for a 1:1 complex of NC and (TG). The grey lines are the profiles expected from fitting the data to a simple 1:1 binding model as described in the Supplementary Data. () Binding of NC to (A). Solutions of NC as in (A) were passed over a 5.8 RU (A) surface. () Binding of NC to a 156.6 RU surface of (TG). Solutions of 1, 2.5, 5, 10, 25, 50, 100, 200, 400, 600, 800 and 1000 nM NC were passed over this surface. The two horizontal lines represent the signals expected if one (lower line) or two (upper line) NC molecules binds to each (TG) molecule. The grey lines are the profiles expected from fitting the data to the complex binding model shown in , as described in the Supplementary Data. () Binding of NC to (TG) in buffer containing 250 mM rather than 150 mM NaCl. Solutions of 5, 10, 25, 50, 100, 200, 400, 800 and 1200 nM NC were passed over a surface containing 159.4 RU of (TG). The horizontal line represents the response expected for a 1:1 complex of NC and (TG). Grey lines are as in (C).
  • Editor: figshare
  • Data de criação/publicação: 2011
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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