Idioma:

Cryogenic Super-Resolution Fluorescence and Electron Microscopy Correlated at the Nanoscale

Dahlberg, Peter D ; Moerner, W. E

Annual review of physical chemistry, 2021-04, Vol.72 (1), p.253-278 [Periódico revisado por pares]

United States: Annual Reviews

Texto completo disponível

Citações Citado por

Enviar para

Título:
Cryogenic Super-Resolution Fluorescence and Electron Microscopy Correlated at the Nanoscale
Autor: Dahlberg, Peter D ; Moerner, W. E
Assuntos: Analysis ; Caulobacter crescentus - ultrastructure ; CLEM ; Context ; Correlation ; Cryoelectron microscopy ; Cryoelectron Microscopy - instrumentation ; Cryoelectron Microscopy - methods ; cryoEM ; cryogenic electron tomography ; Electron Microscope Tomography - instrumentation ; Electron Microscope Tomography - methods ; Electron microscopy ; Fluorescence ; Fluorescence microscopy ; HEK293 Cells ; Humans ; Methods ; Microscopy ; Microscopy, Electron - instrumentation ; Microscopy, Electron - methods ; Microscopy, Fluorescence - instrumentation ; Microscopy, Fluorescence - methods ; Nanotechnology - instrumentation ; Nanotechnology - methods ; Proteins ; Single Molecule Imaging - instrumentation ; Single Molecule Imaging - methods ; State-of-the-art reviews ; Subcellular Fractions - ultrastructure ; super-resolution microscopy
É parte de: Annual review of physical chemistry, 2021-04, Vol.72 (1), p.253-278
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
Descrição: We review the emerging method of super-resolved cryogenic correlative light and electron microscopy (srCryoCLEM). Super-resolution (SR) fluorescence microscopy and cryogenic electron tomography (CET) are both powerful techniques for observing subcellular organization, but each approach has unique limitations. The combination of the two brings the single-molecule sensitivity and specificity of SR to the detailed cellular context and molecular scale resolution of CET. The resulting correlative data is more informative than the sum of its parts. The correlative images can be used to pinpoint the positions of fluorescently labeled proteins in the high-resolution context of CET with nanometer-scale precision and or to identify proteins in electron-dense structures. The execution of srCryoCLEM is challenging and the approach is best described as a method that is still in its infancy with numerous technical challenges. In this review, we describe state-of-the-art srCryoCLEM experiments, discuss the most pressing challenges, and give a brief outlook on future applications.
Editor: United States: Annual Reviews
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 6 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Cryogenic Super-Resolution Fluorescence and Electron Microscopy Correlated at the Nanoscale

Dahlberg, Peter D ; Moerner, W. E

United States: Annual Reviews

Buscando em bases de dados remotas. Favor aguardar.