Idioma:

Hydrogen Sulfide Is an Endogenous Inhibitor of Phosphodiesterase Activity

Bucci, Mariarosaria ; Papapetropoulos, Andreas ; Vellecco, Valentina ; Zhou, Zongmin ; Pyriochou, Anastasia ; Roussos, Charis ; Roviezzo, Fiorentina ; Brancaleone, Vincenzo ; Cirino, Giuseppe

Arteriosclerosis, thrombosis, and vascular biology, 2010-10, Vol.30 (10), p.1998-2004 [Periódico revisado por pares]

Philadelphia, PA: American Heart Association, Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Hydrogen Sulfide Is an Endogenous Inhibitor of Phosphodiesterase Activity
Autor: Bucci, Mariarosaria ; Papapetropoulos, Andreas ; Vellecco, Valentina ; Zhou, Zongmin ; Pyriochou, Anastasia ; Roussos, Charis ; Roviezzo, Fiorentina ; Brancaleone, Vincenzo ; Cirino, Giuseppe
Assuntos: 1-Methyl-3-isobutylxanthine - pharmacology ; Alanine - analogs & derivatives ; Alanine - pharmacology ; Alkynes - pharmacology ; Animals ; Aorta, Thoracic - drug effects ; Aorta, Thoracic - physiology ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Clone Cells ; Cyclic AMP - metabolism ; Cyclic GMP - metabolism ; Cystathionine gamma-Lyase - antagonists & inhibitors ; Cystathionine gamma-Lyase - genetics ; Cystathionine gamma-Lyase - metabolism ; Cysteine - pharmacology ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Endothelial Cells - drug effects ; Endothelial Cells - physiology ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glycine - analogs & derivatives ; Glycine - pharmacology ; Humans ; Hydrogen Sulfide - metabolism ; In Vitro Techniques ; Male ; Medical sciences ; Phosphodiesterase Inhibitors - metabolism ; Rats ; Rats, Wistar ; RNA, Small Interfering - genetics ; Vasodilation - drug effects ; Vasodilation - physiology ; Vertebrates: cardiovascular system
É parte de: Arteriosclerosis, thrombosis, and vascular biology, 2010-10, Vol.30 (10), p.1998-2004
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: OBJECTIVE—Recent studies have demonstrated that hydrogen sulfide (H2S) is produced within the vessel wall from l-cysteine regulating several aspects of vascular homeostasis. H2S generated from cystathione γ-lyase (CSE) contributes to vascular tone; however, the molecular mechanisms underlying the vasorelaxing effects of H2S are still under investigation. METHODS AND RESULTS—Using isolated aortic rings, we observed that addition of l-cysteine led to a concentration-dependent relaxation that was prevented by the CSE inhibitors dl-propargylglyicine (PAG) and β-cyano-l-alanine (BCA). Moreover, incubation with PAG or BCA resulted in a rightward shift in sodium nitroprusside-and isoproterenol-induced relaxation. Aortic tissues exposed to PAG or BCA contained lower levels of cGMP, exposure of cells to exogenous H2S or overexpression of CSE raised cGMP concentration. RNA silencing of CSE expression reduced intracellular cGMP levels confirming a positive role for endogenous H2S on cGMP accumulation. The ability of H2S to enhance cGMP levels was greatly reduced by the nonselective phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. Finally, addition of H2S to a cell-free system inhibited both cGMP and cAMP breakdown. CONCLUSION—These findings provide direct evidence that H2S acts as an endogenous inhibitor of phosphodiesterase activity and reinforce the notion that this gasotransmitter could be therapeutically exploited.
Editor: Philadelphia, PA: American Heart Association, Inc
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Hydrogen Sulfide Is an Endogenous Inhibitor of Phosphodiesterase Activity

Bucci, Mariarosaria ; Papapetropoulos, Andreas ; Vellecco, Valentina ; Zhou, Zongmin ; Pyriochou, Anastasia ; Roussos, Charis ; Roviezzo, Fiorentina ; Brancaleone, Vincenzo ; Cirino, Giuseppe

Philadelphia, PA: American Heart Association, Inc

Buscando em bases de dados remotas. Favor aguardar.