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Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave

Marsolier-Kergoat, Marie-Claude ; Palacio, Pauline ; Berthonaud, Véronique ; Maksud, Frédéric ; Stafford, Thomas ; Bégouën, Robert ; Elalouf, Jean-Marc Orlando, Ludovic

PloS one, 2015-06, Vol.10 (6), p.e0128267-e0128267 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave
Autor: Marsolier-Kergoat, Marie-Claude ; Palacio, Pauline ; Berthonaud, Véronique ; Maksud, Frédéric ; Stafford, Thomas ; Bégouën, Robert ; Elalouf, Jean-Marc
Orlando, Ludovic
Assuntos: Animals ; Bioinformatics ; Bison - classification ; Bison - genetics ; Bison bison ; Bison bison bison ; Bison bonasus ; Bison priscus ; Bone and Bones - metabolism ; Bovidae ; Buffalo ; Carbon Radioisotopes - chemistry ; Caves ; Contaminants ; Contamination ; Deoxyribonucleic acid ; DNA ; DNA sequencing ; DNA, Mitochondrial - analysis ; DNA, Mitochondrial - genetics ; Endangered & extinct species ; Engraving ; Extinction ; Extinction, Biological ; Fossils ; Gene sequencing ; Genome, Mitochondrial ; Genomes ; High-Throughput Nucleotide Sequencing ; Hunting ; Mitochondria ; Mitochondrial DNA ; Nucleotide sequence ; Paleolithic ; Phylogenetics ; Phylogeny ; Pleistocene ; Rib ; RNA polymerase ; Sequence Analysis, DNA ; Soil contamination
É parte de: PloS one, 2015-06, Vol.10 (6), p.e0128267-e0128267
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
Conceived and designed the experiments: JME. Performed the experiments: PP VB TS JME. Analyzed the data: MCMK PP VB TS JME. Contributed reagents/materials/analysis tools: FM RB. Wrote the paper: MCMK JME.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Despite the abundance of fossil remains for the extinct steppe bison (Bison priscus), an animal that was painted and engraved in numerous European Paleolithic caves, a complete mitochondrial genome sequence has never been obtained for this species. In the present study we collected bone samples from a sector of the Trois-Frères Paleolithic cave (Ariège, France) that formerly functioned as a pitfall and was sealed before the end of the Pleistocene. Screening the DNA content of the samples collected from the ground surface revealed their contamination by Bos DNA. However, a 19,000-year-old rib collected on a rock apart the pathway delineated for modern visitors was devoid of such contaminants and reproducibly yielded Bison priscus DNA. High-throughput shotgun sequencing combined with conventional PCR analysis of the rib DNA extract enabled to reconstruct a complete mitochondrial genome sequence of 16,318 bp for the extinct steppe bison with a 10.4-fold coverage. Phylogenetic analyses robustly established the position of the Bison priscus mitochondrial genome as basal to the clade delineated by the genomes of the modern American Bison bison. The extinct steppe bison sequence, which exhibits 93 specific polymorphisms as compared to the published Bison bison mitochondrial genomes, provides an additional resource for the study of Bovinae specimens. Moreover this study of ancient DNA delineates a new research pathway for the analysis of the Magdalenian Trois-Frères cave.
Editor: United States: Public Library of Science
Idioma: Inglês

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Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave

Marsolier-Kergoat, Marie-Claude ; Palacio, Pauline ; Berthonaud, Véronique ; Maksud, Frédéric ; Stafford, Thomas ; Bégouën, Robert ; Elalouf, Jean-Marc Orlando, Ludovic

United States: Public Library of Science

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