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Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells

Ferreira, L. S. ; Diniz, I. M. A. ; Maranduba, C. M. S. ; Miyagi, S. P. H. ; Rodrigues, M. F. S. D. ; Moura-Netto, C. ; Marques, Márcia Martins

Lasers in medical science, 2019-06, Vol.34 (4), p.659-666 [Periódico revisado por pares]

London: Springer London

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Título:
Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells
Autor: Ferreira, L. S. ; Diniz, I. M. A. ; Maranduba, C. M. S. ; Miyagi, S. P. H. ; Rodrigues, M. F. S. D. ; Moura-Netto, C. ; Marques, Márcia Martins
Assuntos: Aluminum ; Biomarkers ; CD105 antigen ; CD90 antigen ; Cell growth ; Cell proliferation ; Continuous radiation ; Data processing ; Dental pulp ; Dentistry ; Flow cytometry ; Gallium ; Gallium indium phosphide ; Indium ; Lasers ; Light therapy ; Medicine ; Medicine & Public Health ; Mesenchyme ; Nestin ; Oct-4 protein ; Optical Devices ; Optics ; Original Article ; Photonics ; Quantum Optics ; Regeneration ; Sheds ; Stem cells ; Teeth ; Therapy ; Tissue engineering ; Variance analysis
É parte de: Lasers in medical science, 2019-06, Vol.34 (4), p.659-666
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: The aim of this in vitro study was to analyze the effect of photobiomodulation therapy (PBMT) on the proliferation and undifferentiating status of stem cell from human exfoliated deciduous teeth (SHEDs). PBMT was carried out with an aluminum gallium indium phosphide (InGaAlP) diode laser in contact and punctual mode (continuous wave, 660 nm, 20 mW, 0.028 cm 2 , and average energy densities of 1 (1 s), 3 (4 s), 5 (7 s), 10 (14 s), 15 (21 s), or 20 (28 s) J/cm 2 per point). The immunoprofile of the SHEDs was analyzed using flow cytometry. Cell proliferation was assessed by the MTT reduction assay. Gene expressions of mesenchymal stem cell markers ( OCT4 , Nestin , CD90 , and CD105 ) were assessed by RT-qPCR 48 h after PBMT. Data were compared by analysis of variance (ANOVA) and Tukey’s test ( p ≤ 0.05). Cells cultured under nutritional deficit and treated with PBMT at 5 J/cm 2 presented similar cell growth than those of positive control group. Cell growth was significantly higher than those of other groups. Mesenchymal stem cell gene markers were still expressed after PBMT at 5 J/cm 2 . In a short-term analysis, PBMT increases the number of stem cells with no interference in the undifferentiated state of the irradiated cells, which opens wide possibilities for application in tissue regeneration.
Editor: London: Springer London
Idioma: Inglês

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Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells

Ferreira, L. S. ; Diniz, I. M. A. ; Maranduba, C. M. S. ; Miyagi, S. P. H. ; Rodrigues, M. F. S. D. ; Moura-Netto, C. ; Marques, Márcia Martins

London: Springer London

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