Idioma:

Cycloheximide can distort measurements of mRNA levels and translation efficiency

Santos, Daniel A ; Shi, Lei ; Tu, Benjamin P ; Weissman, Jonathan S

Nucleic acids research, 2019-06, Vol.47 (10), p.4974-4985 [Periódico revisado por pares]

England: Oxford University Press

Texto completo disponível

Citações Citado por

Enviar para

Título:
Cycloheximide can distort measurements of mRNA levels and translation efficiency
Autor: Santos, Daniel A ; Shi, Lei ; Tu, Benjamin P ; Weissman, Jonathan S
Assuntos: Cycloheximide - pharmacology ; Data Resources and Analyses ; Gene Expression Regulation, Fungal - drug effects ; Meiosis - drug effects ; Meiosis - genetics ; Models, Genetic ; Polyribosomes - metabolism ; Protein Biosynthesis - drug effects ; Protein Synthesis Inhibitors - pharmacology ; Ribosomes - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Saccharomycetales - drug effects ; Saccharomycetales - genetics ; Saccharomycetales - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism
É parte de: Nucleic acids research, 2019-06, Vol.47 (10), p.4974-4985
Descrição: Regulation of the efficiency with which an mRNA is translated into proteins represents a key mechanism for controlling gene expression. Such regulation impacts the number of actively translating ribosomes per mRNA molecule, referred to as translation efficiency (TE), which can be monitored using ribosome profiling and RNA-seq, or by evaluating the position of an mRNA in a polysome gradient. Here we show that in budding yeast, under nutrient limiting conditions, the commonly used translation inhibitor cycloheximide induces rapid transcriptional upregulation of hundreds of genes involved in ribosome biogenesis. Cycloheximide also prevents translation of these newly transcribed messages, leading to an apparent drop in TE of these genes under conditions that include key transitions during the yeast metabolic cycle, meiosis, and amino acid starvation; however, this effect is abolished when cycloheximide pretreatment is omitted. This response requires TORC1 signaling, and is modulated by the genetic background as well as the vehicle used to deliver the drug. The present work highlights an important caveat to the use of translation inhibitors when measuring TE or mRNA levels, and will hopefully aid in future experimental design as well as interpretation of prior results.
Editor: England: Oxford University Press
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Cycloheximide can distort measurements of mRNA levels and translation efficiency

Santos, Daniel A ; Shi, Lei ; Tu, Benjamin P ; Weissman, Jonathan S

England: Oxford University Press

Buscando em bases de dados remotas. Favor aguardar.