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The molecular architecture of the meiotic spindle is remodeled during metaphase arrest in oocytes

Costa, Mariana F A ; Ohkura, Hiroyuki

The Journal of cell biology, 2019-09, Vol.218 (9), p.2854-2864 [Periódico revisado por pares]

United States: Rockefeller University Press

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Título:
The molecular architecture of the meiotic spindle is remodeled during metaphase arrest in oocytes
Autor: Costa, Mariana F A ; Ohkura, Hiroyuki
Assuntos: Animals ; Architecture ; Cell Cycle Checkpoints - physiology ; Crosslinking ; Drosophila melanogaster ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Equator ; Fertilization ; Fruit flies ; Gametocytes ; Kinesin ; Meiosis ; Metaphase ; Metaphase - physiology ; Microtubule-Associated Proteins - genetics ; Microtubule-Associated Proteins - metabolism ; Mitosis ; Oocytes ; Oocytes - cytology ; Oocytes - metabolism ; Proteins ; Spindle Apparatus - genetics ; Spindle Apparatus - metabolism
É parte de: The Journal of cell biology, 2019-09, Vol.218 (9), p.2854-2864
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Before fertilization, oocytes of most species undergo a long, natural arrest in metaphase. Before this, prometaphase I is also prolonged, due to late stable kinetochore-microtubule attachment. How oocytes stably maintain the dynamic spindle for hours during these periods is poorly understood. Here we report that the bipolar spindle changes its molecular architecture during the long prometaphase/metaphase I in oocytes. By generating transgenic flies expressing GFP-tagged spindle proteins, we found that 14 of 25 spindle proteins change their distribution in the bipolar spindle. Among them, microtubule cross-linking kinesins, MKlp1/Pavarotti and kinesin-5/Klp61F, accumulate to the spindle equator in late metaphase. We found that the late equator accumulation of MKlp1/Pavarotti is regulated by a mechanism distinct from that in mitosis. While MKlp1/Pavarotti contributes to the control of spindle length, kinesin-5/Klp61F is crucial for maintaining a bipolar spindle during metaphase I arrest. Our study provides novel insight into how oocytes maintain a bipolar spindle during metaphase arrest.
Editor: United States: Rockefeller University Press
Idioma: Inglês

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The molecular architecture of the meiotic spindle is remodeled during metaphase arrest in oocytes

Costa, Mariana F A ; Ohkura, Hiroyuki

United States: Rockefeller University Press

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