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Simultaneous analysis of natural pigments and E-141i in olive oils by liquid chromatography–tandem mass spectrometry

Arrizabalaga-Larrañaga, Ane ; Rodríguez, Pilar ; Medina, Mireia ; Santos, F. Javier ; Moyano, Encarnación

Analytical and bioanalytical chemistry, 2019-08, Vol.411 (21), p.5577-5591 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Simultaneous analysis of natural pigments and E-141i in olive oils by liquid chromatography–tandem mass spectrometry
Autor: Arrizabalaga-Larrañaga, Ane ; Rodríguez, Pilar ; Medina, Mireia ; Santos, F. Javier ; Moyano, Encarnación
Assuntos: Analytical Chemistry ; Atmospheric pressure ; Beta carotene ; Biochemistry ; Carotene ; Carotenoids ; Cartridges ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chlorophyll ; Chromatography ; Comparative analysis ; Feasibility studies ; Food Science ; High performance liquid chromatography ; Ionization ; Ions ; Laboratory Medicine ; Liquid chromatography ; Lutein ; Mass spectrometry ; Mass spectroscopy ; Monitoring/Environmental Analysis ; Oils & fats ; Olive oil ; Organic chemistry ; Petroleum mining ; Photoionization ; Pigments ; Research Paper ; Scientific imaging ; Silica ; Silicon dioxide ; Solid phases ; Spectroscopy ; Spectrum analysis ; Violaxanthin ; β-Carotene
É parte de: Analytical and bioanalytical chemistry, 2019-08, Vol.411 (21), p.5577-5591
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: This work describes the development of an ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for the determination of carotenoids (β-carotene, lutein, β-criptoxanthin, neoxanthin, violaxanthin) and chlorophylls, as well as their related compounds (chlorophyll A and B, pheophytin A and B and the banned dyes Cu–pyropheophytin A, Cu–pheophytin A and B) in olive oils. For this purpose, the feasibility of electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) for the ionization of these compounds was evaluated and compared. Tandem mass spectrometry (MS/MS) fragmentation was discussed for each family of compounds, and the most characteristic and abundant product ions were selected to propose a selective and sensitive UHPLC–MS/MS method. The best results were obtained using APCI and APPI, while ESI provided the worst signal-to-noise ratio ( S / N ) for all compounds. For the analysis of olive oils, a simple solid-phase extraction (SPE) with silica cartridges was applied before the determination by UHPLC–MS/MS (APCI and APPI) in multiple reaction monitoring (MRM) mode. Method quality parameters were stablished, and the results demonstrate the good performance of the new methods, providing low limits of detection (0.004–0.9 mg L −1 ), high extraction efficiencies (62–95%) and low matrix effects (< 25%). The developed UHPLC–API–MS/MS (APCI and APPI) methods were applied to the analysis of olive oil samples, and β-carotene, pheophytin A, pheophytin B and lutein were detected and quantified in all of them at concentrations ranging from 0.1 to 9.5 mg L −1 . Graphical abstract
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Simultaneous analysis of natural pigments and E-141i in olive oils by liquid chromatography–tandem mass spectrometry

Arrizabalaga-Larrañaga, Ane ; Rodríguez, Pilar ; Medina, Mireia ; Santos, F. Javier ; Moyano, Encarnación

Berlin/Heidelberg: Springer Berlin Heidelberg

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