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Intrapleural transplantation of allogeneic pancreatic islets achieves glycemic control in a diabetic non‐human primate

Lei, Ji ; Zhang, Alexander ; Deng, Hongping ; Yang, Zhihong ; Peters, Cole W. ; Lee, Kang M. ; Wang, Zhenjuan ; Rosales, Ivy A. ; Rickert, Charles G. ; Markmann, James F.

American journal of transplantation, 2022-03, Vol.22 (3), p.966-972 [Periódico revisado por pares]

United States: Wiley Subscription Services, Inc

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Título:
Intrapleural transplantation of allogeneic pancreatic islets achieves glycemic control in a diabetic non‐human primate
Autor: Lei, Ji ; Zhang, Alexander ; Deng, Hongping ; Yang, Zhihong ; Peters, Cole W. ; Lee, Kang M. ; Wang, Zhenjuan ; Rosales, Ivy A. ; Rickert, Charles G. ; Markmann, James F.
Assuntos: animal models: nonhuman primate ; Animals ; Diabetes ; Diabetes mellitus (insulin dependent) ; Diabetes Mellitus, Type 1 - surgery ; Glycemic Control ; Graft Survival ; Hematopoietic Stem Cell Transplantation ; Hypoxia ; Immunological tolerance ; Immunosuppression ; Inflammation ; Islet cells ; islet transplantation ; Islets of Langerhans ; Islets of Langerhans Transplantation - methods ; Oxygen tension ; Pancreas transplantation ; Pancreatic islet transplantation ; Primates ; Survival ; translational research/science ; Transplants & implants
É parte de: American journal of transplantation, 2022-03, Vol.22 (3), p.966-972
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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6. Authors’ Contrbutions
J.L., J.F.M., designed the research and study protocol; J. L. conducted the islet isolation, transplantation, pretransplant and posttransplant animal care with the assistance of A.Z., H.D., Z.Y., C.P., Z.W.; Z.Y., K.L. performed in vitro assays; I.R., J. L. performed histology and immunohistochemical analysis; J. L. analyzed data and wrote the manuscript with the assistance of A.Z., C.P. and C.R.; J. F. M. conducted the islet transplantation, critically reviewed and edited the manuscript.
Descrição: Clinical islet transplantation has relied almost exclusively on intraportal administration of pancreatic islets, as it has been the only consistent approach to achieve robust graft function in human recipients. However, this approach suffers from significant loss of islet mass from a potent immediate blood‐mediated inflammatory response (IBMIR) and a hypoxic environment. To avoid these negative aspects of the portal site, we explored an alternative approach in which allogeneic islets were transplanted into the intrapleural space of a non‐human primate (NHP), treated with an immunosuppression regimen previously reported to secure routine survival and tolerance to allogeneic islets in NHP. Robust glycemic control and graft survival were achieved for the planned study period of >90 days. Our observations suggest the intrapleural space provides an attractive locale for islet transplantation due to its higher oxygen tension, ability to accommodate large transplant tissue volumes, and a lack of IBMIR‐mediated islet damage. Our preliminary results reveal the promise of the intrapleural space as an alternative site for clinical islet transplantation in the treatment of type 1 diabetes. The intrapleural space supports allogeneic islet survival in a diabetic non‐human primate and thus, may represent an alternative site for clinical islet transplantation.
Editor: United States: Wiley Subscription Services, Inc
Idioma: Inglês

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Intrapleural transplantation of allogeneic pancreatic islets achieves glycemic control in a diabetic non‐human primate

Lei, Ji ; Zhang, Alexander ; Deng, Hongping ; Yang, Zhihong ; Peters, Cole W. ; Lee, Kang M. ; Wang, Zhenjuan ; Rosales, Ivy A. ; Rickert, Charles G. ; Markmann, James F.

United States: Wiley Subscription Services, Inc

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