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Molecular analysis of the chromosomal region encoding the nifA and nifB genes of Acetobacter diazotrophicus

Teixeira, Kátia R.S ; Wülling, Manuela ; Morgan, Theodore ; Galler, Ricardo ; Zellermann, Eva-Maria ; Baldani, José I ; Kennedy, Christina ; Meletzus, Dietmar

FEMS microbiology letters, 1999-07, Vol.176 (2), p.301-309 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Molecular analysis of the chromosomal region encoding the nifA and nifB genes of Acetobacter diazotrophicus
Autor: Teixeira, Kátia R.S ; Wülling, Manuela ; Morgan, Theodore ; Galler, Ricardo ; Zellermann, Eva-Maria ; Baldani, José I ; Kennedy, Christina ; Meletzus, Dietmar
Assuntos: Acetobacter diazotrophicus ; Amino acid sequence ; Ammonium ; Azotobacter ; Bacteriology ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Gene sequencing ; Genetics ; Microbiology ; nif gene ; NifA gene ; Nitrogenase ; Oxygen ; Phenotypes ; Proteobacteria
É parte de: FEMS microbiology letters, 1999-07, Vol.176 (2), p.301-309
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: Abstract The Acetobacter diazotrophicus nifA gene was isolated by its ability to restore a Nif+ phenotype to a nifA mutant of Azotobacter vinelandii. Sequencing revealed that the nifA gene was upstream and adjacent to the nifB gene and both are transcribed in the same direction but independently from different promoters. The 3′ end of the nifB gene was located approximately 2.5 kb upstream of the nitrogenase structural gene cluster, nifHDK. The deduced amino acid sequences of the A. diazotrophicus nifA and nifB gene products were most similar to the NifA and NifB proteins of Azorhizobium caulinodans and Rhodobacter capsulatus, respectively. A. diazotrophicus nifA expression was repressed in cultures exposed to high levels of ammonium while oxygen apparently had no influence. Both oxygen and ammonium prevented expression of a nifB-reporter strain, consistent with the observation that ammonium repressed nifA expression, and indicating that A. diazotrophicus NifA activity is inhibited by oxygen as in other Proteobacterial α group diazotrophs.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Molecular analysis of the chromosomal region encoding the nifA and nifB genes of Acetobacter diazotrophicus

Teixeira, Kátia R.S ; Wülling, Manuela ; Morgan, Theodore ; Galler, Ricardo ; Zellermann, Eva-Maria ; Baldani, José I ; Kennedy, Christina ; Meletzus, Dietmar

Oxford, UK: Blackwell Publishing Ltd

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