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Substrate preference of protein kinase B isoforms can vary depending on the cell line

Palma, Miguel S ; Perez, Samantha R ; Husain, Aida ; Bhandari, Deepali

PloS one, 2024-03, Vol.19 (3), p.e0298322-e0298322 [Periódico revisado por pares]

United States: Public Library of Science

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  • Título:
    Substrate preference of protein kinase B isoforms can vary depending on the cell line
  • Autor: Palma, Miguel S ; Perez, Samantha R ; Husain, Aida ; Bhandari, Deepali
  • Assuntos: Analysis ; Biology and Life Sciences ; Cell Line ; Cell membranes ; Health aspects ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; Protein kinases ; Proto-Oncogene Proteins c-akt - metabolism ; Research and analysis methods
  • É parte de: PloS one, 2024-03, Vol.19 (3), p.e0298322-e0298322
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    Competing Interests: The authors have declared that no competing interests exist.
  • Descrição: Many proteins in higher eukaryotes, especially those with crucial functions, have multiple isoforms with redundant roles providing protection against potential functional deficiencies in one isoform. However, these isoforms can also have some unique roles. Protein kinase B, also known as Akt, is one such protein that has three isoforms encoded on different genes. Due to high sequence similarity and the general lack of specific reagents, most studies on Akt generalize their findings and do not distinguish between the isoforms. Using an established chemical genetic strategy and a set of known Akt substrates, this work explores substrate specificity of Akt isoforms under steady state conditions in two commonly used cell lines. This strategy can be applied to study any Akt isoform-specific substrates of interest in any cell line of choice as long as the cell line can be transfected.
  • Editor: United States: Public Library of Science
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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