Idioma:

Immunoassay for human serum hepcidin

Ganz, Tomas ; Olbina, Gordana ; Girelli, Domenico ; Nemeth, Elizabeta ; Westerman, Mark

Blood, 2008-11, Vol.112 (10), p.4292-4297 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Immunoassay for human serum hepcidin
Autor: Ganz, Tomas ; Olbina, Gordana ; Girelli, Domenico ; Nemeth, Elizabeta ; Westerman, Mark
Assuntos: Anemia, Iron-Deficiency - blood ; Anemia, Iron-Deficiency - urine ; Antimicrobial Cationic Peptides - blood ; Antimicrobial Cationic Peptides - urine ; C-Reactive Protein - analysis ; Chronic Disease ; Enzyme-Linked Immunosorbent Assay ; Female ; Ferritins - blood ; Ferritins - urine ; Hemochromatosis - blood ; Hemochromatosis - urine ; Hepcidins ; Humans ; Inflammation - blood ; Inflammation - urine ; Iron ; Kidney Diseases - blood ; Kidney Diseases - urine ; Male ; Multiple Myeloma - blood ; Multiple Myeloma - urine ; Sensitivity and Specificity
É parte de: Blood, 2008-11, Vol.112 (10), p.4292-4297
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: We developed and validated the first serum enzyme-linked immunosorbent assay for hepcidin, the principal iron-regulatory hormone that has been very difficult to measure. In healthy volunteers, the 5% to 95% range of hepcidin concentrations was 29 to 254 ng/mL in men (n = 65) and 17 to 286 ng/mL in women (n = 49), with median concentrations 112 versus 65 (P < .001). The lower limit of detection was 5 ng/mL. Serum hepcidin concentrations in 24 healthy subjects correlated well with their urinary hepcidin (r = 0.82). Serum hepcidin appropriately correlated with serum ferritin (r = 0.63), reflecting the regulation of both proteins by iron stores. Healthy volunteers showed a diurnal increase of serum hepcidin at noon and 8 pm compared with 8 am, and a transient rise of serum hepcidin in response to iron ingestion. Expected alterations in hepcidin levels were observed in a variety of clinical conditions associated with iron disturbances. Serum hepcidin concentrations were undetectable or low in patients with iron deficiency anemia (ferritin < 10 ng/mL), iron-depleted HFE hemochromatosis, and juvenile hemochromatosis. Serum hepcidin concentrations were high in patients with inflammation (C-reactive protein > 10 mg/dL), multiple myeloma, or chronic kidney disease. The new serum hepcidin enzyme-linked immunosorbent assay yields accurate and reproducible measurements that appropriately reflect physiologic, pathologic, and genetic influences, and is informative about the etiology of iron disorders.
Editor: United States: Elsevier Inc
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 15 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Immunoassay for human serum hepcidin

Ganz, Tomas ; Olbina, Gordana ; Girelli, Domenico ; Nemeth, Elizabeta ; Westerman, Mark

United States: Elsevier Inc

Buscando em bases de dados remotas. Favor aguardar.