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The effects of urban particulate matter on the nasal epithelium by gender: An experimental study in mice

Yoshizaki, K. ; Fuziwara, C.S. ; Brito, J.M. ; Santos, T.M.N. ; Kimura, E.T. ; Correia, A.T. ; Amato-Lourenco, L.F. ; Vasconcellos, P. ; Silva, L.F. ; Brentani, M.M. ; Mauad, T. ; Saldiva, P.H.N. ; Macchione, M.

Environmental pollution (1987), 2016-06, Vol.213, p.359-369 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
The effects of urban particulate matter on the nasal epithelium by gender: An experimental study in mice
Autor: Yoshizaki, K. ; Fuziwara, C.S. ; Brito, J.M. ; Santos, T.M.N. ; Kimura, E.T. ; Correia, A.T. ; Amato-Lourenco, L.F. ; Vasconcellos, P. ; Silva, L.F. ; Brentani, M.M. ; Mauad, T. ; Saldiva, P.H.N. ; Macchione, M.
Assuntos: Air Pollutants - analysis ; Air Pollution ; Animals ; Aryl Hydrocarbon Hydroxylases - metabolism ; Aryl hydrocarbon receptor (AhR) ; Cytochrome P-450 CYP1A1 - metabolism ; Cytochrome P-450 CYP1A2 - metabolism ; Cytochrome P-450 CYP1B1 - metabolism ; Cytochrome P450 enzymes ; Estrogen receptor ; Estrogen Receptor beta - metabolism ; Female ; Hydrocarbon polycyclic aromatic ; Male ; Mice ; Mice, Inbred BALB C ; Nasal epithelium ; Nasal Mucosa - drug effects ; Particulate Matter - toxicity ; Polycyclic Aromatic Hydrocarbons - analysis ; Receptors, Aryl Hydrocarbon - metabolism ; RNA, Messenger - metabolism ; Sex Characteristics
É parte de: Environmental pollution (1987), 2016-06, Vol.213, p.359-369
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Nose is the first portion of the respiratory system into contact with air pollution particles, including organic compounds that could act as endocrine releasers. The objective was to identify and quantify estrogenic receptor-β (ERβ), aryl hydrocarbon receptor (AhR), the cytochrome P450 enzymes CYP1A1, 1A2, 1B1, and mucus profile in the nasal epithelium of mice. BALB/c mice male (n = 32) and female (n = 82) in proestrus, estrus and diestrus were divided into two groups: 1) exposed to ambient air; 2) concentrated ambient particles (CAPs) to achieve an accumulated dose (concentration vs. time product) of 600 μg/m3, the time of the exposure was controlled to ensure the same concentration for all groups (5 days per week for 40–51 days). RT-PCR (Erβ-1, Erβ-2, Ahr, Cyp1a1, Cyp1a2, Cyp1b1), immunohistochemistry and morphometry (ERβ, AhR) were used to analyze. The mucus profiles were examined using acid (Alcian Blue) and neutral (periodic acid Schiff's) stains. Exposed females had significantly lower levels of Erβ-2 mRNA than exposed males (p = 0.036). Cyp1b1 mRNA in diestrus females was significantly lower in the CAP-exposed group compared with the ambient air group (p ≤ 0.05). ERβ expression in the epithelium and submucosa nucleus was lower in estrus exposed to CAPs compared with ambient air. CAPs increases AhR in the epithelium (p = 0.044) and submucosa (p = 0.001) nucleus of female when compared with male mice. Exposure to CAPs, also led to relatively increased acidic content in the mucus of males (p = 0.048), but decreased acidic content in that of females (p = 0.04). This study revealed sex-dependent responses to air pollution in the nasal epithelium that may partially explain the predisposition of females to airway respiratory diseases. [Display omitted] •The estrous cycle influences the response of female mice to CAPs in the nasal epithelium.•Exposure to CAPs interferes with the mRNA expression of Erβ-2, Cyp1a2, Cyp1b1 in the nasal epithelium of female mice.•Exposure to CAPs interferes with levels of the receptors of ERβ and AhR in nasal epithelium of female mice.•Male express more nasal acidic and neutral mucus when exposed to CAPs. ERβ gene and protein expression are influenced by estrogen-like and levels of estrogen circulating while AhR is only influenced by estrogen-like in the air pollution.
Editor: England: Elsevier Ltd
Idioma: Inglês

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The effects of urban particulate matter on the nasal epithelium by gender: An experimental study in mice

Yoshizaki, K. ; Fuziwara, C.S. ; Brito, J.M. ; Santos, T.M.N. ; Kimura, E.T. ; Correia, A.T. ; Amato-Lourenco, L.F. ; Vasconcellos, P. ; Silva, L.F. ; Brentani, M.M. ; Mauad, T. ; Saldiva, P.H.N. ; Macchione, M.

England: Elsevier Ltd

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