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Chromosome 8 BAC array comparative genomic hybridization and expression analysis identify amplification and overexpression of TRMT12 in breast cancer

Rodriguez, Virginia ; Chen, Yidong ; Elkahloun, Abdel ; Dutra, Amalia ; Pak, Evgenia ; Chandrasekharappa, Settara

Genes chromosomes & cancer, 2007-07, Vol.46 (7), p.694-707 [Periódico revisado por pares]

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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  • Título:
    Chromosome 8 BAC array comparative genomic hybridization and expression analysis identify amplification and overexpression of TRMT12 in breast cancer
  • Autor: Rodriguez, Virginia ; Chen, Yidong ; Elkahloun, Abdel ; Dutra, Amalia ; Pak, Evgenia ; Chandrasekharappa, Settara
  • Assuntos: Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Cell Line, Tumor ; Chromosomes, Artificial, Bacterial ; Chromosomes, Human, Pair 8 ; Gene Expression Profiling ; Humans ; Nucleic Acid Hybridization ; Oligonucleotide Array Sequence Analysis ; Polymerase Chain Reaction ; tRNA Methyltransferases - genetics
  • É parte de: Genes chromosomes & cancer, 2007-07, Vol.46 (7), p.694-707
  • Notas: ark:/67375/WNG-T6P1PTFX-B
    All of the data from the BAC and expression arrays are deposited at NCBI (http://www.ncbi.nlm.nih.gov/geo/) and the GEO accession number is GSE6567.
    Construction of Chromosome 8 BAC Array and Array CGHIllustration of Segmentation AlgorithmPrimers and Product SizesDifferentially Expressed Genes Derived from Chromosome 8 region of SKBR3 Cell LineDifferentially Expressed Genes from 8 Breast Cancer Cell Lines
    istex:D5701200ED47231D3F4199FF805D3849F7C0BAFB
    ArticleID:GCC20454
    Intramural Research Program of the National Human Genome Research Institute and National Cancer Institute, National Institutes of Health
    All of the data from the BAC and expression arrays are deposited at NCBI
    http://www.ncbi.nlm.nih.gov/geo/
    and the GEO accession number is GSE6567.
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Genomic changes in chromosome 8 are commonly observed in breast cancer cell lines and tumors. To fine map such genomic changes by comparative genomic hybridization (CGH), a high resolution (100 kb) chromosome 8 array that can detect single copy changes was developed using Phi29 DNA polymerase amplified BAC (bacterial artificial chromosome) DNA. The BAC array CGH resolved the two known amplified regions (8q21 and 8q24) of a breast cancer cell line (SKBR3) into nine separate regions including six amplicons and three deleted regions, all of which were verified by Fluorescence in situ hybridization. The extent of the gain/loss for each region was validated by qPCR. CGH was performed with a total of 8 breast cancer cell lines, and common regions of genomic amplification/deletion were identified by segmentation analysis. A 1.2‐Mb region (125.3–126.5 Mb) and a 1.0‐Mb region (128.1–129.1 Mb) in 8q24 were amplified in 7/8 cell lines. A global expression analysis was performed to evaluate expression changes associated with genomic amplification/deletion: a novel gene, TRMT12 (at 125.5 Mb), amplified in 7/8 cell lines, showed highest expression in these cell lines. Further analysis by RT‐qPCR using RNA from 30 breast tumors showed that TRMT12 was overexpressed >2 fold in 87% (26/30) of the tumors. TRMT12 is a homologue of a yeast gene encoding a tRNA methyltransferase involved in the posttranscriptional modification of tRNAPhe, and exploring the biological consequence of its altered expression, may reveal novel pathways in tumorigenesis. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045‐2257/suppmat. Published 2007 Wiley‐Liss, Inc.
  • Editor: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
  • Idioma: Inglês
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