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[ 3H]tryptophan-[ 14C]proline dual label method for the simultaneous determination of collagen and noncollagen protein production

Flaherty, Michael ; Chojkier, Mario

Analytical biochemistry, 1984-11, Vol.142 (2), p.386-394 [Periódico revisado por pares]

San Diego, CA: Elsevier Inc

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Título:
[ 3H]tryptophan-[ 14C]proline dual label method for the simultaneous determination of collagen and noncollagen protein production
Autor: Flaherty, Michael ; Chojkier, Mario
Assuntos: Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Biological Transport ; Bone and Bones - metabolism ; Carbon Radioisotopes ; Cartilage - metabolism ; Chick Embryo ; Collagen - biosynthesis ; collagen synthesis ; collagenase ; dual label ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; proline ; Proline - metabolism ; Protein Biosynthesis ; Proteins ; relative rate ; Tritium ; tryptophan ; Tryptophan - metabolism
É parte de: Analytical biochemistry, 1984-11, Vol.142 (2), p.386-394
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: A new method for the simultaneous determination of newly synthesized collagen and noncollagen proteins has been developed. Because tryptophan is not found in collagen noncollagen proteins were specifically labeled with [ 3H]tryptophan. [ 14C]Proline was used to label both groups of proteins. To calculate the 14C-labeled noncollagen protein the 3H radioactivity of the protein mixture was divided by the ratio of 3H: 14C in noncollagen protein of a representative sample. This value was obtained by collagenase digestion. The remaining 14C radioactivity in the protein mixture was attributed to [ 14C]collagen. There was a very good correlation between the dual label method and the widely used collagenase digestion method for the measurement of collagen and noncollagen protein production and for the calculation of the relative rate of collagen synthesis. This new method provides a simple and accurate analysis of collagen production, and it is suitable for rapid processing of a large number of biological samples.
Editor: San Diego, CA: Elsevier Inc
Idioma: Inglês

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[ 3H]tryptophan-[ 14C]proline dual label method for the simultaneous determination of collagen and noncollagen protein production

Flaherty, Michael ; Chojkier, Mario

San Diego, CA: Elsevier Inc

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