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Genetic Analysis of the rkp-3 Gene Region in Sinorhizobium meliloti 41: rkpY Directs Capsular Polysaccharide Synthesis to K sub(R)5 Antigen Production

Palvoelgyi, A ; Deak, V ; Poinsot, V ; Nagy, T ; Nagy, E ; Kerepesi, I ; Putnoky, P

Molecular plant-microbe interactions, 2009-11, Vol.22 (11), p.1422-1430 [Periódico revisado por pares]

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  • Título:
    Genetic Analysis of the rkp-3 Gene Region in Sinorhizobium meliloti 41: rkpY Directs Capsular Polysaccharide Synthesis to K sub(R)5 Antigen Production
  • Autor: Palvoelgyi, A ; Deak, V ; Poinsot, V ; Nagy, T ; Nagy, E ; Kerepesi, I ; Putnoky, P
  • Assuntos: Sinorhizobium meliloti
  • É parte de: Molecular plant-microbe interactions, 2009-11, Vol.22 (11), p.1422-1430
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    content type line 23
    ObjectType-Feature-1
  • Descrição: Rhizobial surface polysaccharides, including capsular polysaccharides (KPS), are involved in symbiotic infection. The rkp-3 locus of Sinorhizobium meliloti 41 is responsible for the production of pseudaminic acid, one of the components of the K sub(R)5 antigen, a strain-specific KPS. We have extended the sequence determination and genetic dissection of the rkp-3 region to clarify the structure and function of the rkpY gene and to identify additional rkp genes. Except for rkpY, no other genes were found where mutation affected the KPS structure and symbiosis. These mutants show a unique phenotype producing a low molecular weight polysaccharide (LMW PS). Creating double mutants, we have shown that biosynthesis genes of the K sub(R)5 antigen except rkpZ are not necessary for the production of this LMW PS. Polysaccharide analysis of genetically modified strains suggests that rkpY has plei-otropic effects on polysaccharide production. It directs KPS synthesis to the K sub(R)5 antigen and influences lipooligo 3-deoxy-d-manno-2 octulosonic acid (Kdo) production in S. meliloti 41. In addition, rkpY suppresses the lipo-oligoKdo production when it is introduced into S. meliloti 1021.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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