Idioma:

Fetal-maternal interactions in the synepitheliochorial placenta using the eGFP cloned cattle model

Pereira, Flavia Thomaz Verechia ; Oliveira, Lilian J ; Barreto, Rodrigo da Silva Nunes ; Mess, Andrea ; Perecin, Felipe ; Bressan, Fabiana Fernandes ; Mesquita, Ligia Garcia ; Miglino, Maria Angelica ; Pimentel, José RodrigoValim ; Fantinato Neto, Paulo ; Meirelles, Flávio Vieira Muotri, Alysson Renato

PloS one, 2013-05, Vol.8 (5), p.e64399-e64399 [Revista revisada por pares]

United States: Public Library of Science

Texto completo disponible

Citas Citado por

Acciones

Título:
Fetal-maternal interactions in the synepitheliochorial placenta using the eGFP cloned cattle model
Autor: Pereira, Flavia Thomaz Verechia ; Oliveira, Lilian J ; Barreto, Rodrigo da Silva Nunes ; Mess, Andrea ; Perecin, Felipe ; Bressan, Fabiana Fernandes ; Mesquita, Ligia Garcia ; Miglino, Maria Angelica ; Pimentel, José RodrigoValim ; Fantinato Neto, Paulo ; Meirelles, Flávio Vieira
Muotri, Alysson Renato
Materias: Animal genetic engineering ; Animal sciences ; Animal tissues ; Animals ; Animals, Genetically Modified ; Apoptosis ; Biology ; Blood ; Blood plasma ; Cattle ; Cell cycle ; Cell interactions ; Channels ; Chorion ; Chorion - metabolism ; Cloning ; Cloning, Organism ; Coding ; Deoxyribonucleic acid ; DNA ; DNA - metabolism ; Electron microscopy ; Embryo, Mammalian - metabolism ; Embryos ; Endometrium ; Engineering ; Epithelium - metabolism ; Female ; Females ; Fetus - metabolism ; Fetus - ultrastructure ; Fetuses ; Fibroblasts ; Fluorescence ; Food ; Gestation ; Green fluorescent protein ; Green Fluorescent Proteins - metabolism ; Humans ; Immunohistochemistry ; In vitro fertilization ; Leukocytes ; Male ; Maternal effects ; Maternal-Fetal Exchange ; Mice ; Models, Animal ; mRNA ; Nuclear transfer ; Nutrition ; Peripheral blood ; Placenta ; Placenta - metabolism ; Placenta - ultrastructure ; Pregnancy ; Proteins ; RNA ; Rodents ; Surgery ; Transmission electron microscopy ; Ultrasonic imaging ; Uterus ; Veterinary colleges ; Veterinary medicine ; Veterinary Science ; Western blotting
Es parte de: PloS one, 2013-05, Vol.8 (5), p.e64399-e64399
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: FTVP LJO FVM MAM. Performed the experiments: FTVP RSNB FFB LJO LGM JRVP PFN. Analyzed the data: FTVP RSNB AMM FP LJO. Contributed reagents/materials/analysis tools: FVTP LJO MAM FVM FP. Wrote the paper: FTVP AMM LJO RSNB.
Descripción: To investigate mechanisms of fetal-maternal cell interactions in the bovine placenta, we developed a model of transgenic enhanced Green Fluorescent Protein (t-eGFP) expressing bovine embryos produced by nuclear transfer (NT) to assess the distribution of fetal-derived products in the bovine placenta. In addition, we searched for male specific DNA in the blood of females carrying in vitro produced male embryos. Our hypothesis is that the bovine placenta is more permeable to fetal-derived products than described elsewhere. Samples of placentomes, chorion, endometrium, maternal peripheral blood leukocytes and blood plasma were collected during early gestation and processed for nested-PCR for eGFP and testis-specific Y-encoded protein (TSPY), western blotting and immunohistochemistry for eGFP detection, as well as transmission electron microscopy to verify the level of interaction between maternal and fetal cells. TSPY and eGFP DNA were present in the blood of cows carrying male pregnancies at day 60 of pregnancy. Protein and mRNA of eGFP were observed in the trophoblast and uterine tissues. In the placentomes, the protein expression was weak in the syncytial regions, but intense in neighboring cells on both sides of the fetal-maternal interface. Ultrastructurally, our samples from t-eGFP expressing NT pregnancies showed to be normal, such as the presence of interdigitating structures between fetal and maternal cells. In addition, channels-like structures were present in the trophoblast cells. Data suggested that there is a delivery of fetal contents to the maternal system on both systemic and local levels that involved nuclear acids and proteins. It not clear the mechanisms involved in the transfer of fetal-derived molecules to the maternal system. This delivery may occur through nonclassical protein secretion; throughout transtrophoblastic-like channels and/or by apoptotic processes previously described. In conclusion, the bovine synepitheliochorial placenta displays an intimate fetal-maternal interaction, similar to other placental types for instance human and mouse.
Editor: United States: Public Library of Science
Idioma: Inglés

Enlaces

View this record in MEDLINE/PubMed

Volver a la lista de resultados

Anterior Resultado 4 Siguiente Ir a la siguiente página

Realización: Logos de Redes Sociales:

Fetal-maternal interactions in the synepitheliochorial placenta using the eGFP cloned cattle model

United States: Public Library of Science

Buscando en bases de datos remotas, por favor espere