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Functional sperm parameters and fertility of bull semen extended in Biociphos-Plus(R) and Triladyl(R)

Gil, J ; Januskauskas, A ; Haard, M.C ; Haard, M.G.M ; Johanisson, A ; S÷derquist, L ; Rodriquez-Martinez, H

Reproduction in domestic animals, 2000-05, Vol.35 (2), p.69-77 [Periódico revisado por pares]

Oxford, UK: Blackwell Science, Ltd

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  • Título:
    Functional sperm parameters and fertility of bull semen extended in Biociphos-Plus(R) and Triladyl(R)
  • Autor: Gil, J ; Januskauskas, A ; Haard, M.C ; Haard, M.G.M ; Johanisson, A ; S÷derquist, L ; Rodriquez-Martinez, H
  • Assuntos: Biological and medical sciences ; BULLS ; CALIDAD ; CONSERVACION DEL SEMEN ; CONSERVATION DU SPERME ; EGG YOLKS ; ESPERMATOZOO ; FERTILIDAD ; FERTILITE ; FERTILITY ; Fundamental and applied biological sciences. Psychology ; Mammalian reproduction. General aspects ; MOUVEMENT ; MOVEMENT ; MOVIMIENTO ; PLANT PROTEIN ; PROTEINAS VEGETALES ; PROTEINE VEGETALE ; QUALITE ; QUALITY ; SEMEN ; SEMEN PRESERVATION ; SOJA ; SOYBEANS ; SPERMATOZOA ; SPERMATOZOIDE ; SPERME ; TAUREAU ; TORO ; Vertebrates: reproduction ; VIABILIDAD ; VIABILITE ; VIABILITY
  • É parte de: Reproduction in domestic animals, 2000-05, Vol.35 (2), p.69-77
  • Notas: 2000A14228
    L53
    istex:F55358060F3D71F6E2100EC148B2D8701F1901BF
    ark:/67375/WNG-KTP8MVWT-2
    ArticleID:RDA197
  • Descrição: Contents Twenty ejaculates from five dairy AI‐bulls were used to compare, in a split‐sample experiment, the fertility [56 day‐non‐return‐rate (NRR) from more than 14000 AI) and sperm viability post‐thaw of semen diluted with an egg yolk‐ (Triladyl®) or soybean‐based (Biociphos‐Plus®) commercial extender. The in vitro evaluations were divided in two experiments. Experiment 1 (n = 20) included post‐thaw evaluations of motility (subjective and computerized), membrane integrity (CalceinAM/EthD‐1, SYBR‐14/PI, and osmotic resistance test; ORT), and capacitation status (CTC/EthD‐1). Experiment 2 (n = 10) included evaluations of the capacitation‐(CTC/EthD‐1) and acrosome status (FITC‐PSA/EthD‐1) during incubation with/without a challenge with solubilized zona pellucida proteins (SZP). No significant difference in the fertility (69.1 ± 0.8 versus 69.2 ± 0.8) results was found between the two extenders. In experiment 1, the computerized motility evaluations post‐thaw (CASA) showed higher values for Biociphos‐Plus® processed semen for the velocity patterns and lateral sperm head displacement. After 6 h at room temperature (20–22°C) all the CASA motility patterns were significantly higher for Biociphos‐Plus®. The proportion of spermatozoa with intact membranes assessed by CalceinAM was significantly higher in Biociphos‐Plus® (p < 0.001) compared to Triladyl®, but such difference was not seen when using SYBR‐14 or the ORT‐assay. When using the CTC/EthD‐1 assay, a lower proportion of acrosome reacted (AR) spermatozoa post‐thaw (p < 0.01) was found in Biociphos‐Plus® processed semen, as well as a tendency (p < 0.07) for a higher number of uncapacitated spermatozoa. In experiment 2, the proportion of uncapacitated spermatozoa was significantly higher for Biociphos‐Plus® when semen was incubated (38°C and 5% CO2) without SZP at both 0 (p < 0.001) and 30 min (p < 0.05). Concomitantly, Triladyl® showed a higher percentage of capacitated spermatozoa at 0 (p < 0.01), 30 (p < 0.05) and 120 min (p < 0.05). A higher (p < 0.05) incidence of AR‐spermatozoa was seen in Triladyl® at the beginning of the incubation with SZP. No significant difference between extenders was detected for the acrosome status by the FITC‐PSA‐assay. Incubation with SZP induced acrosome reaction of capacitated spermatozoa in both extenders, which was detected by CTC and FITC‐PSA assays. In conclusion, fertility was not affected by Biociphos‐Plus® when 15 × 106 of spermatozoa per AI dose were inseminated. The finding that higher frequencies of spermatozoa seemed more membrane stable post‐thaw, when frozen in Biociphos‐Plus®, might indicate that this extender better protects the sperm viability compared with Triladyl®.
  • Editor: Oxford, UK: Blackwell Science, Ltd
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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