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In vivo protection against Tityus serrulatus scorpion toxins by immunization of mice with a non-toxic protein

Moreira-Ferreira, Ana M.B. ; Kalapothakis, Evanguedes ; Diniz, Carlos R. ; Chávez-Olórtegui, Carlos

Toxicon (Oxford), 1998-02, Vol.36 (2), p.333-339 [Periódico revisado por pares]

Oxford: Elsevier Ltd

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Título:
In vivo protection against Tityus serrulatus scorpion toxins by immunization of mice with a non-toxic protein
Autor: Moreira-Ferreira, Ana M.B. ; Kalapothakis, Evanguedes ; Diniz, Carlos R. ; Chávez-Olórtegui, Carlos
Assuntos: Animal poisons toxicology. Antivenoms ; Animals ; Antibody Formation ; Antivenins - immunology ; Antivenins - isolation & purification ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay ; Female ; Immunization ; in vivo protection ; Lethal Dose 50 ; Medical sciences ; Mice - immunology ; Neutralization Tests ; non-toxic protein ; scorpion toxins ; Scorpion Venoms - antagonists & inhibitors ; Scorpion Venoms - immunology ; Scorpions ; Tityus serrulatus ; Toxicology
É parte de: Toxicon (Oxford), 1998-02, Vol.36 (2), p.333-339
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Descrição: The possibility of inducing a humoral immune response able to produce neutralizing antibodies against the lethal effects of scorpion toxins was evaluated in the mouse model. A non-toxic protein (TsNTxP) was purified from the venom of the Brazilian scorpion Tityus serrulatus by combining gel filtration, ion exchange and reverse phase HPLC chromatographic steps. After four injections of TsNTxP the mice developed an IgG response. The anti-TsNTxP antibodies had a comparable high cross-reactivity for the crude venom, toxic fraction (toxic fraction of venom that represents most of the toxicity of the crude venom — TsTFG50) and TsIV, a representative α-type toxin of T. serrulatus, and moderate binding capacity for TsVII, a representative β-type toxin. In vitro neutralization assays indicated that preincubation of a lethal dose of the toxic fraction with immune serum strongly reduced its toxicity. In vivo protection assays showed that mice immunized with TsNTxP resisted a challenge of 10 ld 50 (s.c.) of the toxic fraction of T. serrulatus venom.
Editor: Oxford: Elsevier Ltd
Idioma: Inglês

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In vivo protection against Tityus serrulatus scorpion toxins by immunization of mice with a non-toxic protein

Moreira-Ferreira, Ana M.B. ; Kalapothakis, Evanguedes ; Diniz, Carlos R. ; Chávez-Olórtegui, Carlos

Oxford: Elsevier Ltd

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