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Superoxide Modulates the Oxidation and Nitrosation of Thiols by Nitric Oxide-derived Reactive Intermediates

Wink, David A. ; Cook, John A. ; Kim, Sungmee Y. ; Vodovotz, Yoram ; Pacelli, Roberto ; Krishna, Murali C. ; Russo, Angelo ; Mitchell, James B. ; Jourd'heuil, David ; Miles, Allen M. ; Grisham, Matthew B.

The Journal of biological chemistry, 1997-04, Vol.272 (17), p.11147-11151 [Periódico revisado por pares]

American Society for Biochemistry and Molecular Biology

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  • Título:
    Superoxide Modulates the Oxidation and Nitrosation of Thiols by Nitric Oxide-derived Reactive Intermediates
  • Autor: Wink, David A. ; Cook, John A. ; Kim, Sungmee Y. ; Vodovotz, Yoram ; Pacelli, Roberto ; Krishna, Murali C. ; Russo, Angelo ; Mitchell, James B. ; Jourd'heuil, David ; Miles, Allen M. ; Grisham, Matthew B.
  • É parte de: The Journal of biological chemistry, 1997-04, Vol.272 (17), p.11147-11151
  • Descrição: Thiol-containing proteins are key to numerous cellular processes, and their functions can be modified by thiol nitrosation or oxidation. Nitrosation reactions are quenched by O 2 , while the oxidation chemistry mediated by peroxynitrite is quenched by excess flux of either NO or O 2 . A solution of glutathione (GSH), a model thiol-containing tripeptide, exclusively yielded S -nitrosoglutathione when exposed to the NO donor, Et 2 NN(O)NONa. However, when xanthine oxidase was added to the same mixture, the yield of S -nitrosoglutathione dramatically decreased as the activity of xanthine oxidase increased, such that there was a 95% reduction in nitrosation when the fluxes of NO and O 2 were nearly equivalent. The presence of superoxide dismutase reversed O 2 -mediated inhibition, while catalase had no effect. Increasing the flux of O 2 yielded oxidized glutathione (GSSG), peaking when the flux of NO and O 2 were approximately equivalent. The results suggest that oxidation and nitrosation of thiols by superoxide and NO are determined by their relative fluxes and may have physiological significance.
  • Editor: American Society for Biochemistry and Molecular Biology
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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