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Sequence analysis of P-genotypes mixtures in bovine rotavirus strains from state of Goiás, Brazil

Thabata Alessandra Ramos Caruzo Maria Lucia Barbosa de Oliveira Rácz; V Munford; W. M. E. D Brito; Congresso Instituto Ciências Biomédicas, IV (2. 2003 São Paulo)

Resumos São Paulo: Comissão de Pesquisa do ICB/USP

São Paulo Comissão de Pesquisa do ICB/USP 2003

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  • Título:
    Sequence analysis of P-genotypes mixtures in bovine rotavirus strains from state of Goiás, Brazil
  • Autor: Thabata Alessandra Ramos Caruzo
  • Maria Lucia Barbosa de Oliveira Rácz; V Munford; W. M. E. D Brito; Congresso Instituto Ciências Biomédicas, IV (2. 2003 São Paulo)
  • Assuntos: MICROBIOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Pesquisa do ICB/USP
  • Notas: Disponível em CD-ROM
  • Descrição: Objetivos:Rotaviruses are the major etiologic agents of acute dehydrating diarrhea in many mammalian species, including calves. Members of the family Reoviridae, they have a genome consisting of 11 segments of dsRNA. The VP4 structural protein, located in the outer capsid, is used for molecular characterization of P genotypes by RT-PCR. The purpose of this work was to partially sequence the gene that encodes VP4 protein for sample BoRV-A/ICB836, previously characterized as P[4][11][6,M37] to confirm RT-PCR characterization and to compare this sample to standard rotavirus ones.Métodos e Resultados: Sequencing of cDNA was performed in an ABI-Prism 3100 DNA Sequencer, with Big Dye kit. Sequences with different sets of primers were analyzed with Lasergene DNAstar software and compared to standard rotavirus strains found in the GenBank, using Blast program. Four different sequences were found for sample BoRV-A/ICB836, showing identities with P[11] BoRV-A/B223 (94.4%); P[4] HuRV-A/RV5 (95.0%), P[5] BoRV-A/UK (89.0%) and P[6] HuRV-A/1076 (95.1%), respectively. Nucleotide sequence analysis of this sample s larger sequence (856nt – P[11]) showed that, at P[4] and P[6,M37]-specific primers binding positions, more than 13 mismatches could be found. Conclusão:These results strongly suggest that this strain may contain four different types of rotaviruses and that a coinfection in the host may have occurred. Also, the presence of two
    typical human P genotypes, P[4] and P[6], in a bovine rotavirus strain in Brazil, suggests that reassortment would be possible among human and bovine rotavirus strains. Finally, the characterization of genotype P[5] only by genomic sequencing shows that this technique is important for a more accurate molecular characterization and more precise information for the development of efficient vaccines against bovine rotaviruses in Brazil
  • Editor: São Paulo Comissão de Pesquisa do ICB/USP
  • Data de criação/publicação: 2003
  • Formato: Poster 259.
  • Idioma: Português
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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