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Pathology; Research findings from Federal University of Sao Paulo, Department of Biophysics update understanding of pathology

Obesity & Diabetes Week, 2010, p.146

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Pathology; Research findings from Federal University of Sao Paulo, Department of Biophysics update understanding of pathology
Assuntos: Oliveira, M
É parte de: Obesity & Diabetes Week, 2010, p.146
Descrição: In pathological processes, cathepsin S is associated with Alzheimer's disease, atherosclerosis and obesity and can be regarded as a potential target in related disorders. However, due to the broad substrate specificities of the lysosomal cathepsins, the specific detection of cathepsin S is difficult when other cathepsins are present. In an attempt to distinguish cathepsin S from other cathepsins we synthesized and tested fluorescence resonance energy transfer (FRET) peptides derived from two of its putative natural substrates, namely insulin beta-chain and class II-associated invariant chain (CLIP). The influence of ionic strength on the catalytic activity and the enzyme stability in neutral pH was also analyzed. Using data gathered from our study we developed a selective substrate for cathepsin S and establish the assay conditions to differentiate the enzyme from cathepsins L, B, V and K.
Editor: Atlanta: NewsRx
Idioma: Inglês

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