Idioma:

Transcriptional changes in U343 MG-a glioblastoma cell line exposed to ionizing radiation

Bassi, CL ; Mello, SS ; Cardoso, RS ; Godoy, PDV ; Fachin, AL ; Junta, CM ; Sandrin-Garcia, P ; Carlotti, CG ; Falcão, RP ; Donadi, EA ; Passos, GAS ; Sakamoto-Hojo, ET

Human & experimental toxicology, 2008-12, Vol.27 (12), p.919-929 [Periódico revisado por pares]

London, England: SAGE Publications

Texto completo disponível

Citações Citado por

Enviar para

Título:
Transcriptional changes in U343 MG-a glioblastoma cell line exposed to ionizing radiation
Autor: Bassi, CL ; Mello, SS ; Cardoso, RS ; Godoy, PDV ; Fachin, AL ; Junta, CM ; Sandrin-Garcia, P ; Carlotti, CG ; Falcão, RP ; Donadi, EA ; Passos, GAS ; Sakamoto-Hojo, ET
Assuntos: Algorithms ; Biological and medical sciences ; Brain cancer ; Brain Neoplasms - genetics ; Brain Neoplasms - pathology ; Brain Neoplasms - radiotherapy ; Cell Line, Tumor ; Cell Survival ; Cellular biology ; Dose-Response Relationship, Radiation ; Gamma Rays ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation, Neoplastic - radiation effects ; Glioblastoma - genetics ; Glioblastoma - pathology ; Glioblastoma - radiotherapy ; Humans ; Medical sciences ; Neurology ; Oligonucleotide Array Sequence Analysis ; Radiation ; Radiation Tolerance - genetics ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; Time Factors ; Transcription, Genetic - radiation effects ; Tumors of the nervous system. Phacomatoses
É parte de: Human & experimental toxicology, 2008-12, Vol.27 (12), p.919-929
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Glioblastoma multiforme (GBM) is a highly invasive and radioresistant brain tumor. Aiming to study how glioma cells respond to γ-rays in terms of biological processes involved in cellular responses, we performed experiments at cellular context and gene expression analysis in U343-MG-a GBM cells irradiated with 1 Gy and collected at 6 h post-irradiation. The survival rate was approximately 61% for 1 Gy and was completely reduced at 16 Gy. By performing the microarray technique, 859 cDNA clones were analyzed. The Significance Analysis of Microarray algorithm indicated 196 significant expressed genes (false discovery rate (FDR) = 0.42%): 67 down-regulated and 97 up-regulated genes, which belong to several classes: metabolism, adhesion/cytoskeleton, signal transduction, cell cycle/apoptosis, membrane transport, DNA repair/DNA damage signaling, transcription factor, intracellular signaling, and RNA processing. Differential expression patterns of five selected genes (HSPA9B, INPP5A, PIP5K1A, FANCG, and TPP2) observed by the microarray analysis were further confirmed by the quantitative real time RT-PCR method, which demonstrated an up-regulation status of those genes. These results indicate a broad spectrum of biological processes (which may reflect the radio-resistance of U343 cells) that were altered in irradiated glioma cells, so as to guarantee cell survival.
Editor: London, England: SAGE Publications
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Transcriptional changes in U343 MG-a glioblastoma cell line exposed to ionizing radiation

Bassi, CL ; Mello, SS ; Cardoso, RS ; Godoy, PDV ; Fachin, AL ; Junta, CM ; Sandrin-Garcia, P ; Carlotti, CG ; Falcão, RP ; Donadi, EA ; Passos, GAS ; Sakamoto-Hojo, ET

London, England: SAGE Publications

Buscando em bases de dados remotas. Favor aguardar.