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Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA

Poinar, Hendrik N ; Schwarz, Carsten ; Qi, Ji ; Shapiro, Beth ; MacPhee, Ross D. E ; Buigues, Bernard ; Tikhonov, Alexei ; Huson, Daniel H ; Tomsho, Lynn P ; Auch, Alexander ; Rampp, Markus ; Miller, Webb ; Schuster, Stephan C

Science (American Association for the Advancement of Science), 2006-01, Vol.311 (5759), p.392-394 [Periódico revisado por pares]

United States: American Association for the Advancement of Science

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Título:
Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA
Autor: Poinar, Hendrik N ; Schwarz, Carsten ; Qi, Ji ; Shapiro, Beth ; MacPhee, Ross D. E ; Buigues, Bernard ; Tikhonov, Alexei ; Huson, Daniel H ; Tomsho, Lynn P ; Auch, Alexander ; Rampp, Markus ; Miller, Webb ; Schuster, Stephan C
Assuntos: Animals ; Base Composition ; Biological taxonomies ; Bones ; Computational Biology ; Cytochromes b - genetics ; Deoxyribonucleic acid ; DNA ; DNA damage ; DNA, Mitochondrial - genetics ; Dogs ; Dogs - genetics ; Elephantidae ; Elephants ; Elephants - genetics ; Endangered & extinct species ; Enzymes ; Fossils ; Gene Library ; Genome ; Genomes ; Genomics ; Humans ; Loxodonta africana ; Mammuthus primigenius ; Mandible - chemistry ; Metagenomics ; Mitochondrial DNA ; Paleontology ; Polymerase Chain Reaction ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Sequencing ; Siberia
É parte de: Science (American Association for the Advancement of Science), 2006-01, Vol.311 (5759), p.392-394
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Descrição: We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.
Editor: United States: American Association for the Advancement of Science
Idioma: Inglês

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Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA

Poinar, Hendrik N ; Schwarz, Carsten ; Qi, Ji ; Shapiro, Beth ; MacPhee, Ross D. E ; Buigues, Bernard ; Tikhonov, Alexei ; Huson, Daniel H ; Tomsho, Lynn P ; Auch, Alexander ; Rampp, Markus ; Miller, Webb ; Schuster, Stephan C

United States: American Association for the Advancement of Science

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