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The optimal time to inject bone mesenchymal stem cells for fracture healing in a murine model

Wang, Xin ; Wang, Cheng ; Gou, Wenlong ; Xu, Xiaolong ; Wang, Yu ; Wang, Aiyuan ; Xu, Wenjing ; Guo, Quanyi ; Liu, Shuyun ; Lu, Qiang ; Meng, Haoye ; Yuan, Mei ; Peng, Jiang ; Lu, Shibi

Stem cell research & therapy, 2018-10, Vol.9 (1), p.272-272, Article 272 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
The optimal time to inject bone mesenchymal stem cells for fracture healing in a murine model
Autor: Wang, Xin ; Wang, Cheng ; Gou, Wenlong ; Xu, Xiaolong ; Wang, Yu ; Wang, Aiyuan ; Xu, Wenjing ; Guo, Quanyi ; Liu, Shuyun ; Lu, Qiang ; Meng, Haoye ; Yuan, Mei ; Peng, Jiang ; Lu, Shibi
Assuntos: Animal models ; Animals ; Benzylamines ; Bone Density ; Bone healing ; Bone marrow ; Bone mineral density ; Bone Regeneration - genetics ; Care and treatment ; Cell Engineering ; Chemokine CXCL12 - antagonists & inhibitors ; Chemokine CXCL12 - genetics ; Chemokine CXCL12 - metabolism ; Chemokines ; Computed tomography ; CXCR4 protein ; Cyclams ; Disease Models, Animal ; Femoral Fractures - diagnostic imaging ; Femoral Fractures - genetics ; Femoral Fractures - pathology ; Femoral Fractures - therapy ; Femur ; Femur - diagnostic imaging ; Femur - injuries ; Femur - metabolism ; Fracture healing ; Fracture Healing - genetics ; Fractures ; Fractures (Injuries) ; Gene Expression ; Health aspects ; Heterocyclic Compounds - pharmacology ; Homing ; Injection ; Laboratory animals ; Laboratory rats ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; Male ; Mechanical properties ; Mesenchymal Stem Cell Transplantation ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - drug effects ; Mesenchymal Stem Cells - metabolism ; Mesenchyme ; Mice ; Mice, Inbred C57BL ; Migration ; Osteogenesis ; Osteogenesis - genetics ; Proteins ; Rats as laboratory animals ; Receptors, CXCR4 - genetics ; Receptors, CXCR4 - metabolism ; Recruitment ; Red Fluorescent Protein ; SDF-1 protein ; Signal Transduction ; Stem cell transplantation ; Stem cells ; Stromal cell-derived factor 1 ; Time Factors ; Transfection ; Transplants & implants ; Vascular endothelial growth factor ; Western blotting ; X-Ray Microtomography
É parte de: Stem cell research & therapy, 2018-10, Vol.9 (1), p.272-272, Article 272
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Descrição: Bone marrow is an important source of stem cells, which can promote bone fracture healing. We investigated the optimal time to inject bone marrow mesenchymal stem cells (BMSCs) in a C57 murine unilateral, transverse, femur fracture model. BMSCs transfected with red fluorescent protein (RFP-BMSCs) were injected via the tail vein on day 1, 7, or 14 post-fracture. AMD3100 (inhibitor of stromal cell-derived factor 1 [SDF-1]) was also injected before RFP-BMSCs in one group for comparison; a control group received saline injections. RFP-BMSC migration and fracture healing were evaluated by in vivo fluorescence assay. Micro-CT was performed and mechanical testing and histological analysis. Chemokine levels were evaluated by quantitative real-time PCR and western blotting. Following injection on day 7 post-fracture, RFP-BMSCs more frequently homed to the fracture site and remained for a longer duration. Bone volume and bone mineral density were increased when BMSCs were injected on day 7 post-fracture (P < 0.05). The mechanical properties of fractured femurs were improved following day-7 BMSC injection. Histology confirmed that BMSC injection improved the formation of new bones. Chemokines that induce BMSC migration were highly expressed, and protein levels of osteogenesis-related factors were increased. Seven days after fracture may be the optimal time for injection of BMSCs to promote fracture healing. Additionally, the SDF-1/CXCR4 pathway may play an important role in fracture healing following BMSC injection.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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The optimal time to inject bone mesenchymal stem cells for fracture healing in a murine model

Wang, Xin ; Wang, Cheng ; Gou, Wenlong ; Xu, Xiaolong ; Wang, Yu ; Wang, Aiyuan ; Xu, Wenjing ; Guo, Quanyi ; Liu, Shuyun ; Lu, Qiang ; Meng, Haoye ; Yuan, Mei ; Peng, Jiang ; Lu, Shibi

England: BioMed Central Ltd

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