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Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans

Garcia, Lana Glerieide Silva; Rocha, Maria Gleiciane Da; Freire, Rosemayre Souza; Nunes, Paulo Iury Gomes; Nunes, Jo��O Victor Serra; Fernandes, Mirele Rodrigues; Sidrim, Jos�� J��Lio Costa; Santos, Flavia Almeida; Rocha, Marcos F��Bio Gadelha; Rodrigues, Lidiany Karla Azevedo; Vieira, Rodrigo Silveira; Brilhante, Raimunda S��Mia Nogueira

Journal of Applied Oral Science; v. 31 (2023); e20230146

Universidade de S��o Paulo. Faculdade de Odontologia de Bauru 2023-10-09

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  • Título:
    Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans
  • Autor: Garcia, Lana Glerieide Silva; Rocha, Maria Gleiciane Da; Freire, Rosemayre Souza; Nunes, Paulo Iury Gomes; Nunes, Jo��O Victor Serra; Fernandes, Mirele Rodrigues; Sidrim, Jos�� J��Lio Costa; Santos, Flavia Almeida; Rocha, Marcos F��Bio Gadelha; Rodrigues, Lidiany Karla Azevedo; Vieira, Rodrigo Silveira; Brilhante, Raimunda S��Mia Nogueira
  • Assuntos: Drug Effects On Biofilms; Dental Plaque; Candida Albicans; Chitosan Analogs & Derivatives; Essential Oils
  • É parte de: Journal of Applied Oral Science; v. 31 (2023); e20230146
  • Descrição: Oral candidiasis is a common fungal infection that affects the oral mucosa, and happens when Candida albicans interacts with bacteria in the oral microbiota, such as Streptococcus mutans, causing severe early childhood caries. C. albicans and S. mutans mixed biofilms are challenging to treat with conventional antimicrobial therapies, thus, new anti-infective drugs are required. Objective: This study aimed to test a drug delivery system based on chitosan microparticles loaded with geranium and lemongrass essential oils to inhibit C. albicans and S. mutans mixed biofilms. Methodology: Chitosan microparticles loaded with essential oils (CM-EOs) were obtained by spray-drying. Susceptibility of planktonic were performed according CLSI at 4 to 2,048 ��g/mL. Mixed biofilms were incubated at 37��C for 48 h and exposed to CM-EOs at 256 to 4,096 ��g/mL. The antimicrobial effect was evaluated using the MTT assay, with biofilm architectural changes analyzed by scanning electron microscopy. RAW 264.7 cell was used to evaluate compound cytotoxicity. Results: CM-EOs had better planktonic activity against C. albicans than S. mutans. All samples reduced the metabolic activity of mixed C. albicans and S. mutans biofilms, with encapsulated oils showing better activity than raw chitosan or oils. The microparticles reduced the biofilm on the slides. The essential oils showed cytotoxic effects against RAW 264.7 cells, but encapsulation into chitosan microparticles decreased their toxicity. Conclusion: This study demonstrates that chitosan loaded with essential oils may provide an alternative method for treating diseases caused by C. albicans and S. mutans mixed biofilm, such as dental caries.
  • DOI: 10.1590/1678-7757-2023-0146
  • Títulos relacionados: https://www.revistas.usp.br/jaos/article/view/216945/198495
  • Editor: Universidade de S��o Paulo. Faculdade de Odontologia de Bauru
  • Data de criação/publicação: 2023-10-09
  • Formato: Adobe PDF
  • Idioma: Inglês
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