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Metabolic engineering of Escherichia coli for the synthesis of polyhydroxyalkanoates using acetate as a main carbon source

Chen, Jing ; Li, Wei ; Zhang, Zhao-Zhou ; Tan, Tian-Wei ; Li, Zheng-Jun

Microbial cell factories, 2018-07, Vol.17 (1), p.102-102, Article 102 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
Metabolic engineering of Escherichia coli for the synthesis of polyhydroxyalkanoates using acetate as a main carbon source
Autor: Chen, Jing ; Li, Wei ; Zhang, Zhao-Zhou ; Tan, Tian-Wei ; Li, Zheng-Jun
Assuntos: 3-Hydroxybutyric Acid - biosynthesis ; 4-Hydroxybutyrate dehydrogenase ; Acetate ; Acetate kinase ; Acetate Kinase - genetics ; Acetates ; Acetates - metabolism ; Acetic acid ; Assimilation ; Bacteria ; Batch Cell Culture Techniques ; Biodiesel fuels ; Biofuels ; Bioplastics ; Biopolymers ; Biopolymers - biosynthesis ; Biosynthesis ; Carbon ; Carbon - metabolism ; Carbon sources ; Cell growth ; Chemical synthesis ; Citric acid ; Cloning ; CoA transferase ; Dehydrogenase ; Dehydrogenases ; E coli ; Engineering ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Feasibility studies ; Fermentation ; Food ; Food consumption ; Genes ; Glucose ; Ketoglutaric acid ; Low cost ; Metabolic Engineering ; Metabolism ; Microorganisms ; Monomers ; Organic chemistry ; Permease ; Phosphate Acetyltransferase - genetics ; Plastics ; Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) ; Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) ; Poly-3-hydroxybutyrate ; Polyhydroxyalkanoates ; Polyhydroxyalkanoates - biosynthesis ; Polyhydroxybutyric acid ; Polymers ; Propionic acid ; Raw materials ; Substrates ; Synthesis ; Synthesis gas ; Yeast ; γ-Hydroxybutyric acid
É parte de: Microbial cell factories, 2018-07, Vol.17 (1), p.102-102, Article 102
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: High production cost of bioplastics polyhydroxyalkanoates (PHA) is a major obstacle to replace traditional petro-based plastics. To address the challenges, strategies towards upstream metabolic engineering and downstream fermentation optimizations have been continuously pursued. Given that the feedstocks especially carbon sources account up to a large portion of the production cost, it is of great importance to explore low cost substrates to manufacture PHA economically. Escherichia coli was metabolically engineered to synthesize poly-3-hydroxybutyrate (P3HB), poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P3HB4HB), and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) using acetate as a main carbon source. Overexpression of phosphotransacetylase/acetate kinase pathway was shown to be an effective strategy for improving acetate assimilation and biopolymer production. The recombinant strain overexpressing phosphotransacetylase/acetate kinase and P3HB synthesis operon produced 1.27 g/L P3HB when grown on minimal medium supplemented with 10 g/L yeast extract and 5 g/L acetate in shake flask cultures. Further introduction succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, and CoA transferase lead to the accumulation of P3HB4HB, reaching a titer of 1.71 g/L with a 4-hydroxybutyrate monomer content of 5.79 mol%. When 1 g/L of α-ketoglutarate or citrate was added to the medium, P3HB4HB titer increased to 1.99 and 2.15 g/L, respectively. To achieve PHBV synthesis, acetate and propionate were simultaneously supplied and propionyl-CoA transferase was overexpressed to provide 3-hydroxyvalerate precursor. The resulting strain produced 0.33 g/L PHBV with a 3-hydroxyvalerate monomer content of 6.58 mol%. Further overexpression of propionate permease improved PHBV titer and 3-hydroxyvalerate monomer content to 1.09 g/L and 10.37 mol%, respectively. The application of acetate as carbon source for microbial fermentation could reduce the consumption of food and agro-based renewable bioresources for biorefineries. Our proposed metabolic engineering strategies illustrate the feasibility for producing polyhydroxyalkanoates using acetate as a main carbon source. Overall, as an abundant and renewable resource, acetate would be developed into a cost-effective feedstock to achieve low cost production of chemicals, materials, and biofuels.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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Metabolic engineering of Escherichia coli for the synthesis of polyhydroxyalkanoates using acetate as a main carbon source

Chen, Jing ; Li, Wei ; Zhang, Zhao-Zhou ; Tan, Tian-Wei ; Li, Zheng-Jun

England: BioMed Central Ltd

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