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Blastocystis subtypes in patients with diabetes mellitus from the Midwest region of Brazil

Melo, Gessica Baptista de ; Mazzaro, Marcia Carolina ; Gomes-Gouvêa, Michele Soares ; Santos, Émelin Alves Dos ; Souza, Laura Vilela de ; Elias-Oliveira, Jefferson ; Gryschek, Ronaldo Cesar Borges ; Rodrigues, Rosângela Maria ; Paula, Fabiana Martins de

Revista do Instituto de Medicina Tropical de São Paulo, 2021-01, Vol.63, p.e32-9 [Periódico revisado por pares]

Brazil: Instituto de Medicina Tropical de Sao Paulo

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  • Título:
    Blastocystis subtypes in patients with diabetes mellitus from the Midwest region of Brazil
  • Autor: Melo, Gessica Baptista de ; Mazzaro, Marcia Carolina ; Gomes-Gouvêa, Michele Soares ; Santos, Émelin Alves Dos ; Souza, Laura Vilela de ; Elias-Oliveira, Jefferson ; Gryschek, Ronaldo Cesar Borges ; Rodrigues, Rosângela Maria ; Paula, Fabiana Martins de
  • Assuntos: Allele ; Blastocystis sp ; Blood tests ; Brazil ; Diabetes ; Diabetes mellitus ; Feces ; Genetic diversity ; Hemoglobin ; Original ; Parasites ; Phylogenetics ; Software ; Subtype ; TROPICAL MEDICINE
  • É parte de: Revista do Instituto de Medicina Tropical de São Paulo, 2021-01, Vol.63, p.e32-9
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    GBM and FMP conceived and designed the study; MCM, EAS, LVS, JEO and RMR collected the samples and carried out the clinical assessment; GBM performed the experiments; GBM, MGG and FMP carried out the analysis and interpretation of these data; GBM, FMP and RCBG drafted the manuscript. All authors read and approved the final manuscript.
    CONFLICT OF INTERESTS
    The authors declare no conflict of interests.
    AUTHORS’ CONTRIBUTIONS
  • Descrição: Blastocystis sp. is an enteric protist commonly found in human fecal samples. In Brazil, few studies have been developed, but none of them has explored the presence of Blastocystis in patients with diabetes mellitus. We evaluated the occurrence and molecular identification of Blastocystis sp. among patients with diabetes mellitus in the Midwest region, Goias State, Brazil. Genomic DNA was obtained from 175 fecal samples (99 from the diabetic group and 76 from the control group). PCR was performed using pan-Blastocystis primers from the SSU-rDNA gene. Microscopic examination revealed positivity of 12.1% and 7.9% for Blastocystis in diabetics and in controls, respectively. Amplification of Blastocystis DNA was observed in 34.4% (34 of 99) and 30.3% (23 of 76) from the diabetic and control groups, respectively. Phylogenetic analyses and BLAST searches revealed six subtypes among Blastocystis isolates in the diabetic group, represented by ST1 (38.2%), ST2 (11.8%), ST3 (35.3%), ST6 (2.9%), ST7 (2.9%) and ST8 (8.8%). In the control group, ST1 (21.8%), ST2 (21.8%), ST3 (43.5%), ST6 (4.4%) and ST8 (8.7%) were identified. This study is the first report regarding the occurrence and subtypes distribution of Blastocystis in patients with diabetes mellitus in Brazil. The results reinforce the potential risk of Blastocystis infection in patients with diabetes, in addition, it contributes to the understanding of the genetic diversity of this enigmatic organism.
  • Editor: Brazil: Instituto de Medicina Tropical de Sao Paulo
  • Idioma: Inglês;Português
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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