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Characterizing VIP Neurons in the Barrel Cortex of VIPcre/tdTomato Mice Reveals Layer-Specific Differences

Prönneke, Alvar ; Scheuer, Bianca ; Wagener, Robin J ; Möck, Martin ; Witte, Mirko ; Staiger, Jochen F

Cerebral cortex (New York, N.Y. 1991), 2015-12, Vol.25 (12), p.4854-4868 [Periódico revisado por pares]

United States: Oxford University Press

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Título:
Characterizing VIP Neurons in the Barrel Cortex of VIPcre/tdTomato Mice Reveals Layer-Specific Differences
Autor: Prönneke, Alvar ; Scheuer, Bianca ; Wagener, Robin J ; Möck, Martin ; Witte, Mirko ; Staiger, Jochen F
Assuntos: Action Potentials ; Animals ; Axons ; Dendrites ; GABAergic Neurons - cytology ; GABAergic Neurons - metabolism ; GABAergic Neurons - physiology ; Immunohistochemistry - methods ; In Situ Hybridization, Fluorescence - methods ; Interneurons - cytology ; Interneurons - metabolism ; Interneurons - physiology ; Mice ; Mice, Transgenic ; Parvalbumins - metabolism ; RNA, Messenger - metabolism ; Somatosensory Cortex - cytology ; Somatosensory Cortex - metabolism ; Somatosensory Cortex - physiology ; Somatostatin - metabolism ; Vasoactive Intestinal Peptide - metabolism
É parte de: Cerebral cortex (New York, N.Y. 1991), 2015-12, Vol.25 (12), p.4854-4868
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Alvar Prönneke and Bianca Scheuer contributed equally to this work.
Jochen F. Staiger and Mirko Witte contributed equally to this work.
Descrição: Neocortical GABAergic interneurons have a profound impact on cortical circuitry and its information processing capacity. Distinct subgroups of inhibitory interneurons can be distinguished by molecular markers, such as parvalbumin, somatostatin, and vasoactive intestinal polypeptide (VIP). Among these, VIP-expressing interneurons sparked a substantial interest since these neurons seem to operate disinhibitory circuit motifs found in all major neocortical areas. Several of these recent studies used transgenic Vip-ires-cre mice to specifically target the population of VIP-expressing interneurons. This makes it necessary to elucidate in detail the sensitivity and specificity of Cre expression for VIP neurons in these animals. Thus, we quantitatively compared endogenous tdTomato with Vip fluorescence in situ hybridization and αVIP immunohistochemistry in the barrel cortex of VIPcre/tdTomato mice in a layer-specific manner. We show that VIPcre/tdTomato mice are highly sensitive and specific for the entire population of VIP-expressing neurons. In the barrel cortex, approximately 13% of all GABAergic neurons are VIP expressing. Most VIP neurons are found in layer II/III (∼60%), whereas approximately 40% are found in the other layers of the barrel cortex. Layer II/III VIP neurons are significantly different from VIP neurons in layers IV-VI in several morphological and membrane properties, which suggest layer-dependent differences in functionality.
Editor: United States: Oxford University Press
Idioma: Inglês

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Characterizing VIP Neurons in the Barrel Cortex of VIPcre/tdTomato Mice Reveals Layer-Specific Differences

Prönneke, Alvar ; Scheuer, Bianca ; Wagener, Robin J ; Möck, Martin ; Witte, Mirko ; Staiger, Jochen F

United States: Oxford University Press

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