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Investigating Protein-Protein Interactions in Multisubunit Proteins: The Case of Eukaryotic RNA Polymerases

Thuriaux, Pierre ; Guglielmi, Benjamin ; Zaros, Cecile ; Werner, Michel Jorde, Lynn B. ; Dunn, Michael J. ; Subramaniam, Shankar ; Little, Peter F. R.

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics, 2005, Vol.5, p.2057-2063

Chichester: John Wiley & Sons, Ltd

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Título:
Investigating Protein-Protein Interactions in Multisubunit Proteins: The Case of Eukaryotic RNA Polymerases
Autor: Thuriaux, Pierre ; Guglielmi, Benjamin ; Zaros, Cecile ; Werner, Michel
Jorde, Lynn B. ; Dunn, Michael J. ; Subramaniam, Shankar ; Little, Peter F. R.
Materias: Cell physiology ; Eukaryotes ; Mapping of Biochemical Networks ; Protein-protein interactions ; RNA polymerases ; Short Specialist Review
Es parte de: Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics, 2005, Vol.5, p.2057-2063
Descripción: The physiological functions executed by eukaryotic cells are often catalyzed by large multiprotein complexes. Very few complexes have been analyzed at a detailed structural level, and one therefore strongly relies on generic but indirect methods to establish protein–protein interaction maps between the polypeptide subunits of these complexes, and to delineate the relevant interacting domains. Currently, the two more popular approaches are based on protein “pull‐down” assays, where the ability of two partner polypeptides is measured by fast copurification tests, and by two‐hybrid interaction mapping, where interacting proteins reconstitute a functional heterodimeric transcriptional activator in vivo. If carefully implemented, both provide important insight into the organization of multiprotein complexes. The predictive power of these approaches will be assessed at the light of our current knowledge of eukaryotic DNA‐dependent RNA polymerases.
Editor: Chichester: John Wiley & Sons, Ltd
Idioma: Inglés

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Investigating Protein-Protein Interactions in Multisubunit Proteins: The Case of Eukaryotic RNA Polymerases

Thuriaux, Pierre ; Guglielmi, Benjamin ; Zaros, Cecile ; Werner, Michel Jorde, Lynn B. ; Dunn, Michael J. ; Subramaniam, Shankar ; Little, Peter F. R.

Chichester: John Wiley & Sons, Ltd

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