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Molecular characterization of bovine rotavirus from São Paulo state, Brazil

V Munford Maria Lucia Barbosa de Oliveira Racz; Congresso Instituto Ciências Biomédicas, IV (2002 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2002

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  • Título:
    Molecular characterization of bovine rotavirus from São Paulo state, Brazil
  • Autor: V Munford
  • Maria Lucia Barbosa de Oliveira Racz; Congresso Instituto Ciências Biomédicas, IV (2002 São Paulo)
  • Assuntos: MICROBIOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002
  • Descrição: Group A bovine rotaviruses (BoRV-A) are among the enteropathogenic agents frequently associated with neonatal diarrhea in calves. Serotype classification of group A rotavirus is based on two differents capsid proteins, VP4 and VP7, which characterize P and G serotypes, respectively. Field surveys demonstrated that, so far, G6 and G10 are the most prevalent G serotypes in calves. The most widespread bovine rotavirus P genotypes are P[1], P[5] and P[11]. Eleven bovine stool specimens were collected from animals with and without diarrhea from São Paulo state. In a previous research, all of the samples were screened for rotavirus by enzyme immunoassay (EIARA-FIOCRUZ) and PAGE. Four of these samples were isolated and propagated in MA-104 and CaCo2 cells, with characteristic cytopathic effect. Additionally, all samples were submitted to the subgroup and serological characterization by using an enzyme immunoassay with monoclonal antibodies (MAb Elisa). In the present study, molecular characterization of these eleven samples was performed using reverse transcription (RT) followed by polimerase chain reaction (PCR) with specific primers for common animal and human G and P genotypes. G genotype results showed five samples with G10 specificity, two with G6 and one with G2 specificity. By serological characterization with Mabs specific for human and animal serotypes of rotavirus, only two of these samples were characterized as G10 For the P type, all samples were
    characterized: 5 samples were P[11] and mixtures of P[4]+P[11], P[8]+P[11], P[1]+P[4], P[5]+P[11] and P[4]+P[5]+P[11] were found in other samples. RT-PCR of two cultivated samples with P[5]+P[11] mixture showed only P[5] specificity, suggesting that cells culture selected one of the strains present in these samples. These results, specially the G[2]P[4] strain, characteristic of human rotavirus, suggests interspecies transmission of rotavirus, and contributes to a better understanding of the epidemiology of bovine and human rotaviruses in Brazil
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2002
  • Formato: 1. (várias paginações) poster 391.; Disponível em CD-ROM.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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