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Drosophila synaptotagmin I null mutants survive to early adulthood

Loewen, Carin A. ; Mackler, Jennifer M. ; Reist, Noreen E.

Genesis (New York, N.Y. : 2000), 2001-09, Vol.31 (1), p.30-36 [Periódico revisado por pares]

New York: John Wiley & Sons, Inc

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Título:
Drosophila synaptotagmin I null mutants survive to early adulthood
Autor: Loewen, Carin A. ; Mackler, Jennifer M. ; Reist, Noreen E.
Assuntos: Animals ; Animals, Genetically Modified ; Behavior, Animal ; Calcium-Binding Proteins - physiology ; Drosophila ; Drosophila - physiology ; Electrophysiology ; exocytosis ; Genes, Lethal ; Immunoblotting ; Immunoenzyme Techniques ; In Vitro Techniques ; Larva - physiology ; Membrane Fusion ; Membrane Glycoproteins - physiology ; Mutagenesis, Site-Directed ; Nerve Tissue Proteins - physiology ; neurotransmission ; Neurotransmitter Agents - secretion ; Phenotype ; Survival Rate ; synapse ; Synapses - physiology ; Synaptotagmin I ; Synaptotagmins
É parte de: Genesis (New York, N.Y. : 2000), 2001-09, Vol.31 (1), p.30-36
Notas: istex:9C6EAF145505C3FBAAD8042B19407F8E4DF5DCC7
ArticleID:GENE10002
March of Dimes
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ObjectType-Article-2
SourceType-Scholarly Journals-1
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Descrição: Synaptotagmin is a synaptic vesicle protein required for efficient neurotransmitter release, yet its exact role in the synaptic vesicle cycle is unclear. Drosophila presents an ideal organism for studies aimed at determining the in vivo functions of proteins. However, synaptotagmin studies have been limited by the early (embryonic or first instar) lethality previously reported for Drosophila synaptotagmin I null (sytnull) mutants. Here we report a new culturing technique that enhances survival of severely uncoordinated mutants thereby permitting Drosophila sytnull mutants to survive through early adulthood. We examined synapses in sytnull third instar larvae by electrophysiology and found that they exhibit severely decreased and asynchronous evoked neurotransmitter release, as well as an increased rate of spontaneous neurotransmitter release, as previously seen in first instar sytnull larvae. The ability to examine severe synaptotagmin mutants as third instar larvae, a stage where electrophysiological and morphological analyses are more easily accomplished, will facilitate structure/function studies. genesis 31:30–36, 2001. © 2001 Wiley‐Liss, Inc.
Editor: New York: John Wiley & Sons, Inc
Idioma: Inglês

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Drosophila synaptotagmin I null mutants survive to early adulthood

Loewen, Carin A. ; Mackler, Jennifer M. ; Reist, Noreen E.

New York: John Wiley & Sons, Inc

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