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Diversity of G2 serotypes/genotypes of human rotavirus in São Paulo, Brazil

V Munford C R Taddei; E S Souza; Edison Luiz Durigon; Maria Lucia Barbosa de Oliveira Racz; Congresso Instituto Ciências Biomédicas, IV (2002 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2002

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  • Título:
    Diversity of G2 serotypes/genotypes of human rotavirus in São Paulo, Brazil
  • Autor: V Munford
  • C R Taddei; E S Souza; Edison Luiz Durigon; Maria Lucia Barbosa de Oliveira Racz; Congresso Instituto Ciências Biomédicas, IV (2002 São Paulo)
  • Assuntos: MICROBIOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002
  • Descrição: Human rotavirus A (HuRV-A) is an important agent of acute gastroenteritis of young children. Three viral proteins are used for characterization of RV-A: VP6 classifies rotavirus into subgroups, VP7 into G serotypes and VP4 into P serotypes/genotypes. We used serological and/or molecular technics for RV-A characterization in São Paulo, Brazil. From 158 stool samples from children with ages between 0 and 5 years, collected from March/94 to September/95, we identified 41 samples with G2 specificity by serotyping with monoclonal antibodies or genotyping by RT-PCR. In 19 samples, G2 serotypes were in agreement with results found by genotyping. In 10 samples, G2 serotype/genotype was found in mixture with other G specificities. From 19 samples with G2 serotype/genotype, 3 had long eletropherotype and subgroup II specificity: 2 were P[8] and one, P[4][8][6]. Ten samples showed short electropherotype, 9 of which was subgroup I and one was subgroup non I-non II. From this, 4 samples were P[4], 3 were P[6], two were P[8] and one samples was negative for P genotyping. Six samples showed a patterns with segment 10 in the usual position and segment 11 positioned very close to it (close electropherotype); all close samples were subgroup I and P[4]. These results shows the diversity of G2 HuRV-A in São Paulo city. These results contributes to a better characterization of rotavirus strains infecting childrens in Brazil and are very important if any vaccination program is intended
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2002
  • Formato: 1. (várias paginações) poster 389.; Disponível em CD-ROM.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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